To clone and express Toxoplasma gondii major surface antigen 1 truncated fragment and analyze its immunological activity.
为构建弓形虫主要表面抗原1(SAG1)的原核重组表达质粒并获得重组表达蛋白,采用聚合酶链反应(PCR)从弓形虫RH株基因组DNA中扩增SAG1基因,经克隆和测序后,亚克隆至原核表达载体pGEX-4T-1,转化大肠杆菌BL21,PCR和双酶切鉴定阳性菌株,表达产物用SDS-PAGE和Western blot进行鉴定观察其诱导的抗体应答。
According to the published gene sequence of the major surface antigen(P30),a pair of primers were designed and synthesized.
弓形虫主要表面抗原Ｐ３０存在于弓形虫表膜及纳虫泡内的一种蛋白，是刺激机体产生免疫反应的主要抗原。
Steel surface should be clean, not a scar on the major surface structures, such as silt dirt.
钢结构表面应干净，结构主要表面不应有疤痕、沙等污垢。
The Clone, Expression and Application of Two Different Fragments of the Major Surface Antigen P30 Derived from Toxoplasma Gondii;
弓形虫主要表面抗原P30两个不同片段的克隆、表达及应用研究
Cloning of the Major Surface Protein P32 Gene from Ovine Theileria and Construction of Eukaryotic Expression Vector
羊泰勒虫主要表面蛋白P32基因的克隆及真核表达载体的构建
depositing plasma
沉积性等离子体（表面改性的主要工艺）
Covering much of the earth's surface is a blanket of water.
地球表面主要被水覆盖着。
much will depend on how they evaluate your interview performance in general.
主要取决于你在面试中总的表现。
Development of Main Application Technique in Surface Non-treatment Cots
表面不处理胶辊主要应用技术的研究
A piece of bread consisting mostly of the hard outer portion.
硬面包片主要由外表硬皮构成的面包片
Carbon dioxide is reported to absorb primarily onto surface alkali or alkali-containing constituents.
据报道CO2吸附主要在碱性表面或含碱成分的表面上。
Lichen is the dominant life-form that covers the surfaces of stones and trees.
青苔是生长在石头和树木表面的主要生物。
Water species adsorb On the surface mainly in the form of undissociated molecules.
水汽主要以不分解的分子态形式吸附在表面。
abrasive roller
磨毛辊（磨毛机的主要部件,辊筒表面包绕砂布带）
These are mainly shan as the narrow coverage rate,the small scale,the single source of the fund,the neak guarantee function,etc.
这主要表现为:覆盖面窄,规模小;基金来源单一;
There are three main ways that Charon's surface supply of crystalline ice could have been replenished.
喀戎表面晶体冰的补给有三种主要的方式。
As pretreatment primer for steel surfaces such as ship and container.
主要用于船舶、集装箱及钢铁表面预处理底漆。
The effect of big family on fertility is stimulating to more birth.
在生育方面，大家庭的影响主要表现在促进多育。
The Important Thoughts of“Three Represents”and the Overall Development of Socialism;
“三个代表”重要思想与社会主义社会全面发展
Three Represents" Completely Embody the Socialist Essential Requirement;
“三个代表”全面体现了社会主义的本质要求