Determination of concentration of ATP by immobilized firefly luciferase reactor;
固定化虫荧光素酶反应器测定 ATP 的初步研究
To assess quickly the potential environmental hazard of heavy metal pollutant by the gene recombinant cell, a reporter vector regulated by electrophile response elements ( EPRE) was constructed by fusing EPRE to the TK minimal promoter and the gene encoding firefly luciferase (LUC) with biomolecular techniques.
研究用基因重组细胞快速评价重金属污染物对健康的危害,采用分子生物学方法,将亲电性化学反应元件(EPRE)与TK基本启动子和编码虫荧光素酶(LUC)基因相融合,构建成EPRE调控的LUC报告载体,将其转染于HeLa细胞。
Study on the Trehalose Protection on Activity of Luciferase;
海藻糖对虫荧光素酶活性保护作用研究
Objective To study the DTA vector containing the Ewing family of tumor s EWS-FLI1 binding sequence depresses the co-transferred luciferase reporter plasmid.
结果 每增加一个 pS2 DTA剂量梯度 ,在尤文肉瘤细胞中都可以检测到虫荧光素酶的表达显著降低 ,而在对照细胞中这种作用却不明显 ;在同一个pS2 DTA剂量梯度下 ,虫荧光素酶在尤文肉瘤细胞的表达都显著高于非尤文肉瘤细胞。
Methods The transfection of luciferase reporter plasmid(pS2),which contains the EWS FLI1 binding sequence,into cultured Ewing′s sarcoma cell lines(RD ES,SK ES) and control cell lines (Hela,NIH3T3)was performed by liposome method and luciferase activity in the cellular lysate was measured by spectrofluorometer.
结果 虫荧光素酶在尤文肉瘤细胞中表达强度明显高于非尤文肉瘤细胞。
The article researches the affect deletion of N terminal amino acids of firefly luciferase on activity of the enzyme.
由于Ｎ末端缺失６个氨基酸的突变体保持了７７％的酶活性，因而萤火虫荧光素酶Ｎ末端第７个氨基酸与酶的催化活性密切相关。
Objective: To study the agreement between chemiluminescence assay(Che) and bioluminescence imaging(Bio) used for detecting the firefly luciferase(Fluc) activity.
目的:比较2种萤火虫荧光素酶活性检测方法的一致性。
In this research,the luc gene coding firefly luciferase(LUC) was amplified by using the DNA of Luciferase reporter vector pXP2 as the template.
以荧光虫荧光素酶报告载体pXP2 DNA为模板,扩增得到编码萤火虫荧光素酶(LUC)的基因(luc),构建了诱导型表达载体pQE30-luc。
A Study on the Properties and Applications of Firefly Luciferase;
萤火虫荧光素酶的性质和应用的研究
Construction of Recombinant Retroviral Vector Containing Firefly Luciferase Reporter Gene
萤火虫荧光素酶报告基因重组逆转录病毒载体的构建及鉴定
Study on the interaction between imidacloprid and humic acid by fluorescence spectroscopy
腐殖酸与吡虫啉作用的荧光光谱研究
Study on the Synthesis and Fluorescent Performance of Coumarin-based Dyes;
香豆素系荧光色素的合成与荧光光谱性能研究
Study on the Determination of Gentamicin and Synchronous Fluorescence Spectrum by Using the Fluorescein Dimer as a Fluorescence Probe
荧光素二聚体荧光探针测定硫酸庆大霉素的同步荧光光谱研究
On summer nights, the glimmering lights are fireflies.
夏日的夜晚荧光点点, 那是荧火虫在飞翔。
isotope X-flue rescent light analyser
同位素Ｘ荧光分析仪
rose Bengal
玫瑰红, 四碘四氯荧光素
tetrachlorotetraiodofluorescein disodium salt
四氯四碘荧光素二钠盐
tetrachlorotetraiodofluorescein dipotassium salt
四氯四碘荧光素二钾盐
radionuclide fluorescence analysis
放射性核素荧光分析
Detection and Identification of Bursaphelenchus Xylophilus with Real-time Fluorogenic Polymerase Chain Reaction;
松材线虫实时荧光PCR检测技术的研究
Development of LUX PCR detection assay for Perkinsus infection of mollusks
贝类派琴虫LUX荧光PCR检测方法的建立
A comparison of methods in observation Bursaphelenchus xylophilus by fluorescent microscope
松材线虫荧光显微观察方法比较研究
Determination of Amikacin by Synchronous Fluorescence Technique with Fluorescein Dimer as a Probe
荧光素二聚体荧光探针同步荧光法测定阿米卡星
Fluorimetric Determination of Copper Ion in Water With 4,6-Dibromofluorescein;
4,6-二溴荧光素荧光法测定微量铜离子的研究
Application of Wave Scan Technology to Discriminate Fluorescent Siderophore and Fluorescin;
波长扫描在鉴别荧光铁载体与荧光素中的应用
Study on the Fluorescence Character of Fluorescein With Anionic Surfactant
荧光素与阴离子表面活性剂的荧光性质研究