Secreted Expression of Mature Peptide Gene of Human Bone Morphogenic Protein-7 in Pichia pastoris;
人骨形成蛋白-7成熟肽基因在巴斯德毕氏酵母中的分泌表达
Cloning and Expressing of gsh 1 gene in Pichia pastoris;
gsh 1基因的克隆及其在巴斯德毕赤氏酵母中的表达
Expression of Monellin, a Sweet Protein, in Pichia pastoris;
甜蛋白Monellin在巴斯德毕赤氏酵母中的胞内表达
Objective:Construction of one novel recombinant Pichia pastoris for production of glutathione,and to study its glutathione synthesis capacity.
目的:构建一株新型的产谷胱甘肽的重组巴斯德毕赤氏酵母,并研究该重组菌合成谷胱甘肽的能力;方法:利用PCR技术克隆来源于酿酒酵母的γ-L-谷氨酰-L-半胱氨酸合成酶编码基因GSH1和谷胱甘肽合成酶的基因GSH2,串联插入表达载体pGAPZA,转化Pichia pastoris GS115,在Zeo-cin平板上挑选阳性转化子,并对阳性转化重组菌进行摇瓶培养筛选。
An image is firstly segmented into approximately homogeneous polygon regions by a split and merge process, and then a refinement procedure is conducted, which minimizes the variation in gray level values within each Voronoi region by adjusting the position of its seed, so that boundaries between adjacent Voronoi regions can run parallel to the c.
通过分割过程将图像粗略分割成灰度分布均匀的多边形区域 ,优化过程通过调整V氏图种子点的位置 ,使得各个V氏图区域中的灰度变化达到极小 ,以使相邻V氏图区域的边界能够吻合图像的轮廓 。