Objective:To evaluate two-dimensional gel electrophoresis(2DGE)and mass spectrometry in the studies of the serum proteins expressed in patients with BPH and those with high-grade prostatic intraepithelial neoplasm(HGPIN).
目的:探讨双向凝胶电泳和质谱技术在前列腺高级别上皮内瘤(HGPIN)和良性前列腺增生(BPH)患者血清蛋白质表达研究中的应用。
Objective To perform the proteome analysis of conditioned medium prepared from mouse embryonic fibroblast feeder layers by 2-dimensional (2D) electrophoresis and mass spectrometry and to find out the possible differentiation-inhibitory factor in conditioned medium.
目的:运用双向电泳、质谱技术对小鼠胚胎成纤维细胞饲养层条件培养液进行蛋白质组学分析,寻找 其中可能具有抑制分化作用的蛋白因子。
Albumin-globulins,glutenins and gliadins of grains were separated by different extraction methods,and analyzed by 2-DE,MS or MS/MS),SDS-PAGE or reversed-phase high-performance liquid chromatography(RP-HPLC).
采用不同的蛋白提取方法,分别利用双向电泳(2-DE)和生物质谱技术(MS)、SDS-PAGE电泳以及反相高效液相色谱(RP-HPLC)等主要蛋白质组技术进行了鉴定与分析,初步得到了三种蛋白的合成与积累特征,为今后小麦发育蛋白质组学以及品质改良研究提供了科学依据。
Albumin-globulins,glutenins and gliadins of grains were separated by different extraction methods,and analyzed by 2-DE,MS or MS/MS),SDS-PAGE or reversed-phase high- performance liquid chromatography(RP-HPLC).
采用不同的蛋白提取方法,分别利用双向电泳(2-DE)和生物质谱技术(MS)、SDS-PAGE 电泳以及反相高效液相色谱(RP-HPLC)等主要蛋白质组技术进行了鉴定与分析,初步得到了三种蛋白的合成与积累特征, 为今后小麦发育蛋白质组学以及品质改良研究提供了科学依据。
By citing a few typical examples of practical problem solving with the use of helium mass spectrometry technology this paper expounds the latter s application in turbogenerator leakage detection and recommends a series of specific techniques for its effective use.
应用氦质谱技术解决实际问题的几例典型例子 ,阐述了氦质谱技术在汽轮发电机组中的用途及其中的一系列应用技巧。
collection angle
收集角 -二次离子质谱术
sensitivity factor
灵敏度因子(二次离子质谱术)
useful ion yield
有用离子产额-二次离子质谱术
interference signal
干扰信号 -二次离子质谱术
incident particle energy
入射粒子能量 -二次离子质谱术
negative ion yield
负离子产额 -二次离子质谱术
The Application of Chromatography Coupled with Electrospray Mass Spectrometry in Analysis of Proteins;
色谱质谱联用技术在蛋白质分析鉴定中的应用
The application of LC-MS/MS in measurement of oxidative stress parameters
液相色谱-质谱/质谱联用技术在氧化应激指标测定中的应用
Progress in the Preprocessing of Mass Spectrometry Proteomics Data
蛋白质组学质谱数据预处理技术综述
Study on Sample Introduction Techniques for Special Samples in Gas Chromatographic/Mass Spectroscopic Analysis;
特殊样品预处理与气相色谱/质谱在线联用技术
Electrothermal Vaporization Atomic Spectrometry/Mass Spectrometry for Trace Analysis;
电热蒸发原子光谱/质谱联用技术及痕量分析
Restricted Access Chromatography, Mass Spectrometry Technology and Their Application in Pharmaceutical Analysis;
限进色谱及质谱技术在药物分析中的应用研究
Application of Liquid Chromatography -Mass Spec- trometry in Forensic Toxicology;
液相色谱—质谱联用技术在毒物分析中的应用
A Review for the Applications of Chromatography-mass Spectrometry in Food Analysis
色谱-质谱联用技术在食品分析中的应用
Applications of chromatography-mass spectrometry in metabonomics
色谱-质谱联用技术在代谢组学中的应用
Study on Determanation of Multi-Pesticide Residues Using Gas Chromatography Mass Spectrometry and Gel Permeation Chromatography
凝胶渗透色谱—气相色谱/质谱技术在农药多残留分析中的应用
mass analyzed ion kinetic energy spectrometry
质量分析离子动能度谱术
Mass spectrometer plays a key role of modern analytical technology.
质谱是现代分析技术皇冠上的明珠。