Methods: The experimental models of proliferation of cultured smooth muscle stimulated by endothelin(ET) were established and cell account and 3H-thymidine(~3H-TdR) incorporation were used.
方法培养人脐动脉平滑肌细胞,应用ET-1建立平滑肌增殖模型,后加入TMPz,设立梯度浓度的TMPz分组,最后细胞计数以及氚-胸腺嘧啶核苷(3H—TdR)掺入方法检测药物效应。
MethodsThe experiment models of proliferation of cultrured smooth muscle stimulated by oxidized low density lipoprotein(ox-LDL)were established and cell account and ~3H-thymidine(~3H-TdR)incorporation were used.
方法培养人脐动脉平滑肌细胞,应用ox-LDL建立平滑肌增殖模型,后加入TMPz,设立梯度浓度的TMPz分组,最后细胞计数以及氚-胸腺嘧啶核苷掺入方法检测药物效应。