To clarify the roles of nuclear localization signal (NLS) in inhibitory effect of HSP70 on H2O2-mediated nucleolar segregation, four eukaryotic vectors pcDNA3.
过氧化氢处理后3h,可见明显的核仁分离。
The cones of clone 28 can aggrandize normally, but their ovules aborted due to the free nuclei mitosis failure of female gametophyte in the seed orchard of Pinus tabulaeformis Carr.
为进一步分析该不育系游离核停止分裂的原因,本论文在系统研究油松28号不育系游离核停止分裂前后细胞形态学和组织化学的基础上,以油松种胚为材料建立了油松悬浮细胞系,采用细胞同步化技术、流式细胞仪技术、蛋白质双向电泳技术等研究手段初步分析了油松细胞周期调控机制;并进一步利用免疫印迹、免疫组化技术探讨了细胞周期相关调控因子在游离核分裂关键时期的表达。