The dominant-active mutant p160 ROCK-ⅠΔ3 was transfected into the 4 cell lines mediated by LipofectamineTM 2000.
方法RT-PCR及Western blot检测RhoC及ROCK-Ⅰ在卵巢癌细胞SW626、Skov-3、A2780和Caov-3中mRNA及蛋白的表达水平;将携带ROCK-Ⅰ组成性激活突变体p160ROCK-ⅠΔ3的质粒(pCAG-myc-p160ROCK-ⅠΔ3)以脂质体介导,转染上述4株人卵巢癌细胞,划痕试验和Boyden小室检测转染前后4种卵巢癌细胞的体外迁移与侵袭能力的变化,MTT比色法测定p160ROCK-ⅠΔ3对细胞增殖能力的影响。
Influence of gap,impurities and component mutation on dynamic character of functionally graded structures;
空隙、杂质及组分突变对功能梯度构件动力特性的影响
constitutive mutant
组成性突变[使基因得到组成型表达的突变]
a mutation due to an intramolecular reorganization of a gene.
一个基因分子内部进行带基本性质的重新组合所形成的突变。
An evaluation of the changes in soft and hard tissues in adult and adolescent with bimaxillary protrusion following treatment with MBT pre-adjusted technique and tooth extraction
MBT技术矫治成年与青少年双颌前突软硬组织改变及相关性分析
Production and Functional Analysis of Recombinant Human Soluble BAFF and Its Mutants;
重组人可溶性BAFF及其突变体的制备与活性分析
Characteristics of Low Phytic Acid Maize Mutant and Effect of Nitrogen on Phytic Acid Content and Phosphorous Composition in Maize;
玉米低植酸突变体的主要特性及N素水平对植酸含量与P素组成的影响
Studies of the Biological Activities of the Mutant Recombinant Human Zona Pellucida Protein 3(mrhZP3);
突变重组人卵透明带蛋白3的生物活性研究
Preparation and Functional Analysis of Recombinant Human Soluble APRIL and Its Mutants;
重组人可溶性APRIL及其突变体的制备与功能分析
Aberrance of Fragile Histidine Triad Gene in Patients with Lung Cancer;
人肺癌脆性组氨酸三联体基因突变的研究
Definition of Catastrophe of Nonlinear One-dimensional Function of Organizational Scope of Enterprises;
非线性单主元企业组织规模发展的突变分析
The Defining of the Catastrophe of Nonlinear One-Dimensional Function of Organizational Scope of Enterprises;
非线性单主元企业组织规模发展的突变点判定
Relationship between JAK2V617F mutation and thrombopoiesis in patients with chronic myeloprolifrative neoplasms
慢性骨髓增殖性肿瘤JAK2V617F突变与血栓形成的关系
DNA-negative mutant
ＤＮＡ－负性突变型
Research of NPM1 Gene Mutations in Adult Patients with Acute Myeloid Leukemia;
成人急性髓系白血病患者NPM1基因突变的研究
Study the Relativity between Ala601Thr Mutation of Plasminogen and Thrombosis;
纤溶酶原Ala601Thr突变与血栓形成相关性研究
A Comparative Study on the Mutagenicity of Natural Borneol and Synthetic Borneol;
天然冰片与合成冰片的致突变性比较研究
Social Change Model in Russia: Analysis on Formation Roots of Sudden Change;
俄罗斯“突变性”社会转型模式生成根源分析
TGF-β1 Expression and Histological Changes in Condyle in Response to Bilateral Mandibular Distraction Osteogenesis of Canine;
狗双侧下颌牵张成骨中髁状突的组织结构变化和TGF-β1的表达
Soft-tissue and hard-tissue profile changes following orthodontic treatment of bimaxillary protrusion in adults with teeth extraction
成人双颌前突错畸形患者拔牙正畸治疗后颅面软硬组织的变化