Application of protein transduction in gene therapy;
蛋白转导在基因治疗中的应用
An update review on a new promising biotechnology:protein transduction;
一种新兴的富有生命力的生物技术——蛋白转导
Objective:To study the effects of the brain-blood barrier permeable PTD-BDNF(protein transduction domain-brain derived neurotrophic factor) on the spontaneous neural discharge in the neurons of rat hippocampus.
方法:应用全细胞膜片钳技术,观察脑源性神经营养因子(BDNF)和蛋白转导结构域-脑源性神经营养因子(PTD-BDNF)对原代培养大鼠海马神经元自发突触活动的影响。
Recent progress in internalization mechanism of protein transduction domain;
蛋白转导域内在化机制的研究进展
The cationic 11-amino acid fragment derived from human immunodeficiency type 1 (HIV-1) tat protein, termed TAT protein transduction domain, has proven to be an invaluable tool to deliver a wide variety of macromolecules across the plasma membrane and into intact tissues.
TAT蛋白转导域是源自人类免疫缺陷病毒Tat蛋白的一段碱性氨基酸多肽,能够将与之共价连接的多肽、蛋白、核酸等生物大分子快速而高效地转导入细胞内部,在药物转运和疾病治疗等领域有着巨大的应用潜力。
Through the medium of protein transduction domain(PTD) composed by a small number of alkaline peptides, the functional proteins linked covalently with DNA, peptides, proteins or other macromolecules can across the membranes of mammalian cells, even the blood-brain barrier and transfer unconventionally that isn t like classical pathway.
蛋白转导是近几年生命科学领域发现的一种独特现象,具有蛋白转导功能的蛋白通过几个短的碱性小肽组成的蛋白转导域(proteintransductiondomain,PTD)的介导,能将与其共价连接的DNA、多肽或蛋白质以及其他大分子物质通过非经典途径穿过细胞膜甚至血脑屏障,并在细胞间自由传递。
The term "protein transduction domain" (PTD) is typically used to refer to a class of small (<20 amino acid) cationic peptides that can transport large, biologically-active molecules into a population of mammalian cells, which serves as a useful tool in the study on protein functions.
蛋白质转导结构域(protein transduction domain,PTD)是指一类能将其他生物大分子导入多种哺乳动物细胞的小分子阳离子多肽,它可以用于研究细胞内某些蛋白质的功能,还可以与具有治疗作用的蛋白质、多肽等生物大分子偶联,在缺血、肿瘤等多种疾病的治疗中有潜在的应用价值。
Protein transduction or protein therapy, as an important approach of disease therapy, is achieved through linking exogenous therapeutic proteins to the fragment of amino acid, termed as protein transduction domain, using the expression vector or chemical cross-linking method.
蛋白质转导或蛋白质治疗是通过表达载体法或化学交联法将具有治疗作用的外源性蛋白质与称为蛋白质转导域的氨基酸片段相连,以非受体和非温度依赖的方式越细胞膜进入胞内达到治疗疾病的目的。
The fused gene (TAT PTD-Ngb) which included nine amino acid transactivator of transcription (TAT) protein transduction domain (RKKRRQRRR) of HIV-1 and neuroglobin gene was amplified by RT-PCR from rat brain RNA and cloned into the expression plasmid pET28b.
以SD大鼠脑组织RNA为模板,利用RT-PCR技术,将HIV-1反式激活蛋白(TAT)中具有蛋白质转导功能的9个氨基酸序列,即蛋白质转导域(PTD)基因与脑红蛋白(Ngb)基因融合,应用T-A克隆技术将融合基因与pMD19-Tsimple载体连接,经测序正确后克隆至表达载体pET28b中,转化感受态E。
Studies on Protein Transduction of VP22 in CVI988 Attenuated Vaccine Strain of Marek's Disease Virus;
MDV CVI988弱毒疫苗株VP22蛋白转导功能研究
Advances in Study on HIV-Tat Protein Transduction Domain
HIV-Tat蛋白转导域的研究进展
The Study of the Transmembrane Activity of the PTD of Tat Protein Fused at the Carboxyl-terminus of Heterologous Protein;
融合在外源蛋白羧基端的Tat蛋白转导结构域的跨膜递送作用研究
Prokaryotic Expression and Biological Activity of Poly-Arg Protein Transduction Domain-Apoptin Fusion Protein
多聚精氨酸蛋白转导域-凋亡素融合蛋白的原核表达及其生物活性
Pax4 protein can be transduced into mammalian cells
转录因子Pax4蛋白质转导功能的研究
Functional Analyses on Regulator of G Protein Signaling (RGS) Proteins in Arabidopsis Thaliana;
拟南芥G蛋白信号转导调节蛋白（AtRGS1蛋白）功能研究
Proteomics Analysis Reveals Insight into the Mechanism of H-Ras-Mediated Transformation;
H-Ras介导细胞转化的蛋白质组学分析
Pro-Apoptotic Protein-BclGs Inhibits Ap-1 Activity Through Jab1;
促凋亡蛋白（BclGs）的信号转导机制研究
Transformation of Gossypium Hirsutum Al-induced (GhAlin) Gene;
棉花铝诱导蛋白基因（GhAlin）的遗传转化
Study on Protein Transduction and Immunogenicity of BCR/ABL Fusion Protein Mediated by Protein Transduction Domain;
PTD介导BCR/ABL蛋白细胞转导及免疫原性的研究
The Signal Transduction Role of Ca~(2+)/Calmodulin-Dependent Protein Kinase Ⅱ in Cardiac Remodeling
Ca~(2+)/钙调蛋白依赖性蛋白激酶Ⅱ在心脏重构中的信号转导作用
Tat transduction peptide displayed on the nucleocapsid improved the baculovirus transduction of mammalian cells
核衣壳蛋白VP39融合TAT转导肽对杆状病毒转导哺乳动物细胞的影响
Functional Analysis of a Novel Family of Protein Kinases;
一种新型蛋白激酶信号转导系统的初步研究
Screening for the Signal Transduction Molecule-PAK4 Binding Proteins by Yeast Two Hybridization;
信号转导分子PAK4相互作用蛋白质的筛选
The Signal Transduction Pathways of Imidazoline-1 Receptor Candidate Protein;
咪唑啉1型受体候选蛋白信号转导的研究
Study on Transformation of NHX Gene into Maize;
Na～+/H～+逆向转运蛋白基因导入玉米的研究
Introduction of hsp101 Gene into Kentucky Bluegrass Mediated by Agrobacterium Tumefaciens;
农杆菌介导法将热激蛋白基因（hsp101）转入早熟禾
The Relationship of G Protein and H_2O_2 in Guard Cell Signal Transduction;
G蛋白和H_2O_2在保卫细胞信号转导中的关系