Establishment and identification of the CHO cell lines which express attenuated mutants of Shiga-like toxin Ⅰ;
稳定表达突变减毒志贺样毒素Ⅰ的CHO细胞株的建立与鉴定
Shiga-like toxin typeⅡvariant (SLT-Ⅱe) is composed of one A subunit and five B subunits.
利用重叠延伸PCR法对志贺样毒素 型变异体A亚单位(SLT- eA)基因进行修饰扩增,将第167位编码Glu的密码子突变为Gln的密码子,第170位编码Arg的密码子突变为编码Lys密码子,并用质粒载体pGEX-6P-1在大肠杆菌BL21中进行融合表达,在不改变其蛋白空间构象的同时降低其毒性。
Objective To construct a gene of ScFv against recombinant Shiga like toxin 2B and express it in Ecoli.
目的构建重组志贺样毒素亚Ⅱ结合亚单位(Stx2B)单链抗体基因,并在大肠杆菌中进行分泌表达。
Class 1 Integron in Shiga toxin-producing Esherichia coli Strains:Its Identification and Analysis;
产志贺样毒素大肠埃希菌Ⅰ类整合子的鉴定和分析
Objective To explore the distribution and characterization of Shiga toxin-producing E.
目的探讨不同标本中产志贺样毒素大肠埃希菌(STEC)的分布特征。
Objective:To construct recombinant replication defective adenoviral vectors encoding 1/100 and 1/1000 attenuated Shiga-like toxinⅠ(Stx1) gene,and to study their therapeutic effects against breast cancer T47D cells in vivo.
目的:构建携带1/100、1/1000减毒活性的突变减毒志贺样毒素Ⅰ(Shiga-like toxin1,Stx1)编码序列的复制缺陷型腺病毒,并观察其抗人乳腺癌细胞裸鼠移植瘤的活性。
Basic Study on Application of Attenuated Mutants of Shiga-like Toxin Ⅰ in Cancer Therapy;
突变减毒志贺样毒素Ⅰ用于抗肿瘤的应用基础研究
The Experimental Study on Engineering Antibody of Anti-EHEC O157:H7 Shiga Like Toxin Ⅱ;
抗肠出血性大肠杆菌O157:H7志贺样毒素Ⅱ基因工程抗体的实验研究
Study on Antibiotic Resistance Mechanism of Enterohaemorrhagic E.coli and Shiga-toxin Producing E.coli;
肠出血性及产志贺样毒素大肠埃希菌耐药机制的研究
Preparation of Monoclonal Antibodies Against B Subunit of Shiga-like Toxin Ⅱ Variant;
志贺样毒素Ⅱ型变异体B亚基单克隆抗体的制备
Detection of shiga toxin 2 gene by ramification amplification method
网状分枝扩增方法检测志贺毒素2基因
Cloning, Expression and Antibodies Preparation of Shiga Toxin A, B Genes;
志贺毒素A、B亚单位基因的克隆表达及抗体制备
Cloning and Expression of Shiga Toxin Gene and Research of the Immunity of Shiga Toxin;
志贺毒素基因克隆表达及其免疫学活性研究
A multiplex PCR for detection of Shiga Toxin-producing Escherichia coli isolated from ducks
多重PCR方法检测鸭源产志贺氏毒素大肠杆菌
Monoclonal Antibody S2C4 Neutralizes The Toxicity of Shiga Toxin 2 and Its Variants
单克隆抗体S2C4对2型志贺毒素及其亚型毒性的中和作用(英文)
Study on Molecular Mechanisms of Multiple Antimicrobial Resistance among Shiga Toxin-producing Escherichia Coli;
产志贺毒素大肠埃希菌多重耐药分子机制的研究
Rapid Gold Immunochromatography Assays for Detection of E.Coli O157 and Shiga Toxin;
免疫胶体金技术快速检测大肠杆菌O157和志贺毒素
Identification of an Insertion Sequence, IS1203 Variant, in a Shiga Toxin 2 Gene of Escherichia Coli O157:H7 in China;
我国发现携带插入序列IS1203变种的志贺毒素2基因的大肠杆菌O157：H7
Preparation Monoclonal Antibody of Anti-Shiga Toxin ⅡB and Using for Immunoaffinity Chromatography;
抗志贺毒素ⅡB亚基（Stx2B）单抗的研制及其亲和层析的应用
Study on Shiga Toxin Ⅱ e Variant and Ricin Induced Apoptosis of Vero Cell;
大肠杆菌志贺毒素Ⅱ型e突变体和蓖麻毒素诱导Vero细胞凋亡的研究
Detection of six kinds of virulence genes in Shigella species by multiplex PCR
志贺菌6种毒力基因的多重PCR检测
Study on the Virulent Genotype and Viruence of Shigella in Lanzhou City;
兰州市区志贺菌毒力基因型及毒力研究
The Prevalence of the Cytolethal Distending Toxin Genes in Chinese Escherichia Coli and Shigella Strains;
细胞致死性肿胀毒素基因在我国大肠杆菌和志贺氏菌中的分布研究
The pathogenicity of Shigella isolates with different virulence genotypes demonstrated in Lanzhou city
兰州市不同毒力基因型志贺菌致病力研究