Construction and expression detection of eukaryotic expression vector of humanized green fluorescent protein gene;
人源化绿色荧光蛋白基因真核表达载体的构建与表达检测
Cloning,sequencing of human interleukin 10 cDNA and construction of its eukaryotic expression vector;
人白细胞介素-10 cDNA的克隆、测序和真核表达载体的构建(英文)
We conducted the following series of studies:Sichuan duck typeⅠinterferon IFN-αORF gene sequence was cloned、identified and analysis;IFN-αORF prokaryotic gene expression vector and eukaryotic expression vector were successful constructed; interferon-αhas been expressed by prokaryotic expression &.
本文对四川省内地方品种四川麻鸭的干扰素进行如下系列研究:对四川麻鸭Ⅰ型干扰素基因IFN-αORF进行克隆、鉴定和序列分析;构建IFN-αORF基因原核表达载体和真核表达载体;IFN-α原核表达及其生物学活性研究;利用雏鸭为模型,应用免疫组织化学方法研究pcDNA-SDIFN-α真核表达质粒在鸭体内的动态表达分布规律;同时对作为免疫佐剂的pcDNA-SDIFN-α真核表达质粒免疫调节活性进行研究,获得以下结果:1。
Expression of lumbrokinase genes in eukaryotic cell COS-7;
蚓激酶基因在真核细胞COS-7中的表达
The eukaryotic expressing vector of glycoprotein was constructed and added with CpG motifs from Hantaan virus that could strengthen mouse immune stimulation,in order to detect whether or not it could express in the eukaryotic cell.
构建汉滩病毒G2糖蛋白的真核表达载体,并加入可增强小鼠免疫刺激作用的CpG基序,检测其可否在真核细胞中表达。
Objective: To construct eukaryotic expression vector of adenovirus type 5 fiber gene and investigate its expression in eukaryotic cell.
目的 :构建腺病毒纤维蛋白基因的真核表达质粒 ,并检测其在真核细胞中的表达 ,为腺病毒靶向性载体构建创造条件。
Construction of eukaryotic expression vector of siRNA specific for osteopontin and characterization of its efficiency in GC9811 gastric carcinoma cell line;
Osteopontin特异性siRNA真核表达载体的构建及其在GC9811胃癌细胞中沉默效应的鉴定
Objective: To construct the eukaryotic expression vector pcDNA3.
目的:构建人甲状腺转录因子-1(TTF1)编码基因的真核表达载体pcDNA3。
Construction and identification of bicistronic eukaryotic expression vector pVAX1-IRES for gene coexpression;
双顺反子真核表达载体pVAX1-IRES的构建与鉴定
Eukaryotic expression and identification of human pSecTag2B-CD226 fusion protein;
人pSecTag2B-CD226真核表达载体的构建、表达及初步鉴定
Construction and expression of HSC70 gene eukaryotic expression plasmid;
人HSC70基因真核表达载体的构建和表达
Improving method of rapid purification for eukaryotic cells total RNA;
快速纯化真核细胞总RNA方法的改良
Fugene 6——a new approach to induce the gene transfection in high efficiency for eukaryotic cells in v itro;
Fugene6——一种介导体外真核细胞高效率基因转染的新方法
Methods:Expression plasmids of hDaxx and 6 △hDaxx in the eukaryotic cells were constructed in pM1 or pM2.
目的 构建N端融合有Gal4DBD的hDaxx及 6种删除突变体 (△hDaxx)真核细胞表达质粒 ,并在HT1 0 80细胞中表达。