The phycocyanin intergenic spacer region (PC-IGS) and microcystin synthetase gene B (mcyB) were identified by whole-cell PCR in water samples,and the amplified.
方法从2004年3月开始,采集西流湖水体表层水样,用血细胞计数板法计数藻细胞;设计特异引物,采用全细胞PCR方法检测水样中藻青蛋白基因中间序列(PC-IGS)和微囊藻毒素多肽合成酶基因mcyB,并对mcyB扩增产物克隆测序。