The leaves of Cattleya hybrida were used as experiment materials and the influences of pretreatment time,enzyme combinations,digestion time and mannitol concentrations in the mixed enzyme solution on protoplast isolation from them were studied.
试验以卡特兰叶片组织为材料,研究了预处理时间、酶液组合、酶解时间、酶液中甘露醇含量等不同因素对其原生质体分离的影响。
The results showed that the time of the protoplast isolation was prolonged for 2 hours when TSFEC had been cultured in media with Hygromycin,but the protoplast output and livability were little affected.
以荔枝优良品种下番枝转gus和hpt基因悬浮细胞再生的松散性胚性愈伤组织(TSFEC)为试材,进行原生质体分离研究及初步培养。
incana seedlings,osmotic stabilizer,different combinations of enzyme mixture and digesting time,on protoplast isolation from the hypocotyls of M.
以紫罗兰Matthiola incana无菌苗下胚轴为材料,研究了光照条件、无菌苗日龄、酶液渗透压、酶液组合及酶解时间对其原生质体分离效果。
Studies on Callus Induction and Protoplast Isolation on Ziziphus Jujuba Mill;
枣愈伤组织诱导和原生质体分离研究
Advances on Isolation and Fusion of Plant Subprotoplasts
植物亚原生质体分离及融合研究进展
Conditions Optimization of Arabidopsis Mesophyll Protoplast Isolation
拟南芥叶肉原生质体分离条件的优化
Establishment of in Vitro Regeneration System and Protoplast Isolation of Orange-Heading Chinese Cabbage;
橙色大白菜离体再生体系的建立及原生质体分离
Study on Regeneration System from Leaf in Vitro and Protoplast Isolation and Culture of Black Chokeberry;
黑果腺肋花楸离体叶片再生体系建立与原生质体分离培养研究
Study on Protoplast Isolation and Electrofusion of Poplar;
杨树原生质体分离及电融合技术体系的研究
The Skill of Hazel Mushroom and Pine Mushroom Protoplasm s Separation and Regeneration;
蜜环菌和松蕈原生质体分离与再生的技术
Protoplast isolation and plant regeneration from leaves of Rhodiola sachalinensis
高山红景天叶肉原生质体分离培养与植株再生
Protoplast Isolation of Callus in Echinacea augustifolia
紫锥菊愈伤组织原生质体分离方法初探
Studies on the Protoplast Separation, Purification and Culture of Gladiolus;
唐菖蒲原生质体分离、纯化及培养的研究
Establishment of Synchronous Suspension Culture and Isolation of Microprotoplasts in Citrus;
柑橘同步化悬浮系建立及微原生质体分离
Studies on the Technique of the Isolation, Purification and Culture of Protoplast in Apple;
苹果原生质体分离、纯化及培养技术研究
The Study on Primary Factors Influencing the Protoplast Culture and Isolation in Sugarcane;
影响甘蔗原生质体分离、培养因素的研究
Study on Protoplast Isolation of Embryogenic Cell Suspensions of Musa acuminata cv.Mas(AA)
贡蕉胚性悬浮细胞原生质体分离的研究
Studies on Callus Induction and Protoplast Isolation on Ziziphus jujuba Mill.cv.Dongzao
冬枣花药愈伤组织的诱导及原生质体分离
1.Isolation and Culture of Protoplasts of Physcomitrium Sphaericum 2.Studies on RNAi System in Tobacco;
1.立碗藓原生质体分离及培养的研究 2.烟草中RNAi体系的研究
Isolation of Mesophyll Protoplast from Arabidopsis Thaliana and the Setup of Transient Expression Assay System
拟南芥叶肉原生质体分离及瞬时表达体系的建立
Influences of Seedling Age and Red Light on Protoplast Isolation and Culture of Sunflower
苗龄与红光对向日葵原生质体分离和培养的影响