The results showed: (1)It was more rapid of the method of co-amplification than hybridization to produce the heteroduplex DNA;(2)The PCR product could be used as the substrate directly;(3)The mutation detection effect was improved by lowing the reaction temperature or shortening the reaction time or improving the concentration of the NaCl in the buffer properly.
优化了S1核酸酶突变检测体系,结果表明(1)扩增法比杂交法产生异源双链DNA更节省时间;(2)PCR产物可以直接作为突变检测的底物;(3)适当降低反应温度、适当增加反应缓冲液中的NaCl浓度、适当缩短反应时间可提高突变检测效果。
Objective Using bacteriophage M13mp2 and its derivatives to construct two kinds of heteroduplex DNA molecular serving as templates for mismatch repair, with one possessing single base mismatching and the other possessing two bases deletion.
目的：噬菌体M13mp2特殊的生活史决定了每一种噬菌体有双链和单链两种DNA形式，以其为材料构建含有单碱基错配的异源双链DNA分子，用于检测由hMSH2-hMSH6(hMutSα)识别错配启动的修复过程；以及含有双碱基缺失的异源双链DNA分子，用于检测主要由hMSH2-hMSH3(hMutSβ)识别插入-缺失环(insertion-deletion loop，IDL)启动的修复过程。
Methods The microsatellite polymorphism of MICA exon 5 in 175 unrelated healthy individuals were investigated using PCR heteroduplex analysis.
方法 用 PCR-异源双链分析法 ,对 175名正常无关个体的MICA基因第 5外显子微卫星多态性的分布进行研究。