Tumor cell-specific gene transfer with retroviral vectors displaying single-chain antibody;
单链抗体-包膜蛋白嵌合型单嗜性逆转录病毒构建及靶向感染肿瘤细胞的研究(英文)
Methods Recombinant retroviral vector pLNCX/iNOS was transferred into the amphotropic packaging cell line PA317 by lipofectamine.
方法 用PCR技术从 pCMV/iNOS质粒中扩增出iNOS全长cDNA ,与逆转录病毒pLNCX构建重组质粒 pLNCX/iNOS ;通过脂质体介导把重组质粒转染入包装细胞PA3 17中 ,经G418筛选出抗性克隆。
Methods Full length cDNA of iNOS were amplified by PCR from pCMV/iNOS vector with retroviral vectors to construct pLNCXiNOS.
方法 :用PCR技术从pCMV/iNOS质粒中扩增出iNOS全长cDNA ,与逆转录病毒pLNCX构建重组质粒pLNCX/iNOS ;通过脂质体介导把重组质粒转染入包装细胞PA3 17中 ,经G418筛选出抗性克隆。
Construction and identification of recombinant defective retroviral vector with mutant human proinsulin gene and stable virus-producing cell lines;
人胰岛素原突变体重组逆转录病毒及稳定包装细胞系的构建和鉴定
Antitumor Effected by Retroviral Expressing wtp53 Gene Driven by hTERT Promoter;
hTERT启动子调控p53基因表达逆转录病毒的抗肿瘤作用
The titer of retroviral was estimated by Hela.
目的 建立产逆转录病毒的PA31 7/HGSTπ细胞株 ,为进一步把耐药基因GSTπ导入造血干细胞以逆转卵巢癌化疗药物对骨髓的毒副作用的研究奠定基础。
xenotropic retrovirus
异嗜性逆转录病毒[只能在异种细胞而不能在原始宿主细胞引起产毒性感染]
amphotropic retrovirus
兼嗜性逆转录病毒，双嗜性逆转录病毒[既能在宿主细胞又能在异种细胞上引起产毒性感染]
ecotropic retrovirus
亲嗜性逆转录病毒[只能在原始宿主细胞引起产毒性感染]
Infection risk of porcine endogenous retrovirus in xenotransplantation
猪内源性逆转录病毒在异种移植中的感染风险
Biological theory and experimental foundation for transduction of cultured Schistosoma japonicum cells with amphotropic retrovirus
双嗜性逆转录病毒感染日本血吸虫细胞的生物学理论与可行性探讨
Evaluation of Biologic Safety with Porcine Endogenous Retrovirus in Xenotransplantation;
异种移植中的猪内源性逆转录病毒生物安全性评价研究
A Preliminary Study on Biologic Safety with Porcine Endogenous Retrovirus in Xenotransplantation;
猪内源性逆转录病毒异种移植生物安全性的初步研究
Construction and Validation of Skeletal Muscle-Specific Reporter Gene Retroviral Vector;
骨骼肌特异性报告基因逆转录病毒载体的构建与验证
highly active antiretroviral therapy (HAART)
高效抗逆转录病毒疗法
INHIBITION OF AVIAN INFLUENZA VIRUS REPLICATION BY RETROVIRUS-MEDIATED SPECIFIC ANTISENSE RAN AND RIBOZYME;
逆转录病毒介导的特异性反义RNA和核酶抑制禽流感病毒复制的研究
The Study of Molecular Characterization of Porcine Endogenous Retrovirus and the Selection of Specific Antiviral Sites;
猪内源性逆转录病毒分子特性的研究及抗病毒位点的选择
Hepatotoxicity associated with antiretroviral therapy containing non-nucleosides reverse transcriptase inhibitors in HIV/AIDS patients
艾滋病患者抗逆转录病毒治疗的肝毒性特点分析
Construction of the Bcr/abl Retroviral Vector-Mediated Murine Model of Chronic Myeloid Leukemia;
bcr/abl逆转录病毒介导的小鼠慢性粒细胞白血病样模型的建立
Detection of rubella virus gene sequences by RT-nested PCR
用逆转录套式PCR检测风疹病毒RNA
avian myeloblastosis virus reverse transcriptase
禽成髓细胞瘤病毒逆转录酶
Construction and Identification of Retroviral Vector Expressing TH Gene
重组逆转录病毒pLNCX2-TH的构建及鉴定
Study on Association between Human Endogenous Retrovirus and Sporadic Amyotrophic Lateral Sclerosis
人内源性逆转录病毒与散发型肌萎缩侧索硬化相关性研究
Transcription of antisense RNA of hepatitis B virus through retroviral mediated gene transfer
逆转录病毒载体介导乙型肝炎病毒反义基因的转录表达