Methods: Decellular porcine aortic heart valve leaflets, were precoated with fibronectin(Fn),carboxy-terminal cell-binding domain of fibronectin(FnCTD_(64)),bovine serum albumin(BSA) for 12 h separately; the leaflets in control group were not precoated.
方法:去细胞猪主动脉瓣叶分别用纤维连接蛋白(Fn)和重组纤维连接蛋白羧基端细胞结合域(FnCTD64)及牛血清白蛋白(BSA)浸泡预涂处理12 h,对照组未预涂蛋白。