Effect of silicon(Si) on alleviation of cadmium(Cd) toxicity to rice seedlings grown hydroponically under the toxic levels of Cd was investigated with special reference to the effect on Cd distribution,chemical forms of Cd,peroxidase activity and the relation with silica-binding protein.
通过水培试验,主要从硅对镉的分布、镉的化学形态和过氧化物酶活性的影响以及与硅结合蛋白的关系等方面研究了硅减轻镉毒害的机制。
In order to detect the distribution of silica-binding proteins in rice and other graminaceous plants, specific polyclonal antibodies against a silica-binding protein from rice, namely SBP117, are successfully raised by synthesized peptides which are conjugated with Keyhole Limpet Hemocyanin and used as antigens to immunize rabbits.
为了检测硅结合蛋白在水稻和禾本科植物体内的分布,根据硅结合蛋白(silica-binding protein 117,SBP117)的氨基酸保守片段和天然抗原表位,合成了SBP117的两个肽段做抗原,与匙孔血蓝蛋白载体偶联后免疫兔子,获得了抗SBP117的专一性抗体。
The paper was the first time to report the method of isolating and extracting the silica-binding protein, its molecular character, the function and its distribution.
本论文首次报道了水稻叶片中硅结合蛋白的分离提取方法、分子特征及其功能与分布,从分子水平上揭示了水稻控制硅沉积的机制,并进一步探讨了硅结合蛋白与硅减轻水稻重金属镉毒害的关系。