E2 genes with different lengths of transmembrane region(TMR) of Hog cholera lapinised virus(HCLV) were amplified by RT-PCR,and cloned into pGEX-4T-1 to construct recombinant plasmid pGEXTE2-339, pGEXTE2-355, pGEXTE2-375, respectively.
猪瘟病毒E2蛋白C端含有一段30多个疏水性氨基酸组成的跨膜区域(Transmembraneregion,TMR),用RTPCR和巢式PCR分别扩增了含不同长度TMR的猪瘟兔化弱毒E2基因,并克隆入pGEX4T1的MCS中,构建了原核表达载体pGEXTE2339(无TMR)、pGEXTE2355(1TMR)、pGEXTE2375(3TMR)。
The Bovine Viral Diarrhea/Mucosal Disease is caused by Bovine Viral Diarrhea virus/Mucosal Disease virus (BVDV/MDV), which characterized by fever, ulcers, and desquamation in mucosal of digestive tract, hemorrhagic inflammation, diarrhea, transient leukopenia and reproductive failure.
本研究首先通过对四川部分地区奶牛、牦牛病毒性腹泻／粘膜病进行了流行病学研究和病原分离鉴定，再依据牛病毒性腹泻／粘膜病病毒与猪瘟病毒在免疫学上具有同源性的特点，介绍了猪瘟兔化弱毒苗的研制和生产过程，并用其对不同种属和日龄的牛进行了不同剂量的免疫，分别从免疫应答及其对牛的生产性能和安全性等方面进行了研究，旨在为用猪瘟兔化弱毒苗预防和控制牛病毒性腹泻／粘膜病提供理论依据。
Expression of the Gene Encoding the A/D Antigenic Domains of the E2 Glycoprotein of HCLV in Escherichia Coli and Preliminary Use of the Recombinant Protein;
猪瘟兔化弱毒编码E2囊膜糖蛋白A/D抗原区基因的原核表达和重组蛋白的初步应用
The Research about the Virus Preserver of Hog Cholera Lapinised Virus (C Strain) and Marek S Disease Turkey Herpes Virus (HVT);
猪瘟兔化弱毒（C株）和鸡马立克火鸡疱疹病毒（HVT）病毒保护剂的研究
Molecular Cloning of Infectious Full-length cDNA of Attenuated Lapinized CSFV Chinese-strain (Derived from Spleen);
中国猪瘟兔化弱毒（脾淋毒）全长感染性cDNA的分子克隆
The probing of a potential method distinguishing the Attenuated Hog Cholera Virus Lapinized from the wild type virus of HCV;
一个潜在的可区分猪瘟兔化弱毒和野毒方法的探索
Cloning and Sequence Analysis on E2 Gene of Different Cell Strains Infected by Hog Cholera Virus Lapinized Chinese Strain;
猪瘟兔化弱毒疫苗株细胞毒E2基因的克隆与序列分析
Expression and Purification of Hog Cholera Lapinised Virus E~(rns) Gene in Escherichia coli
猪瘟兔化弱毒株E~(rns)基因在大肠埃希氏菌中的表达、纯化
Construction of the cDNA Clone and Chimeric Clones of Classical Swine Fever Virus C-strain Vaccine;
猪瘟兔化弱毒疫苗株cDNA分子克隆与嵌合分子克隆的构建
Comparison of Cell Immunity Efficiency between HCLV Bovine Testis Cell Vaccine and HCLV Rabbit Spleen and Lymph Tissue Origin Vaccine
猪瘟兔化弱毒细胞苗和脾淋苗细胞免疫效果的比较
Development of a TaqMan fluorogenic quantitative RT-PCR assay for rapid quantification of hog cholera lapinized virus
快速检测猪瘟兔化弱毒疫苗株的TaqMan荧光定量RT-PCR方法的建立
Expression of the Gene Encoding the A/D Antigenic Domains of the E2 Glycoprotein of HCLV in Escherichia Coli and Preliminary Use of the Recombinant Protein;
猪瘟兔化弱毒编码E2囊膜糖蛋白A/D抗原区基因的原核表达和重组蛋白的初步应用
Establishment of RT-Nested PCR Detection Method about Classical Swine Fever Virus and Study on Immunity and Eradication of CSF in Breeding Swine Field
猪瘟病毒套式RT-PCR检测方法的建立和种猪场猪瘟免疫与净化研究
Study on the E2 Gene Nucleotide Sequences of Classical Swine Fever Virus Field Strains and Their Potential Molecular Difference with the Attenuated Classical Swine Fever Virus Lapinized;
猪瘟分离毒E2基因序列分析和强弱毒检测方法的探讨
Visualized detection of wild-type classical swine fever virus using RT-LAMP
猪瘟病毒野毒株RT-LAMP可视化检测方法的建立
Expression of E2 Gene of Classical Swine Fever Virus Shimen Strain on Immortalised Swine Vein Endothelial Cells;
猪瘟病毒石门株E2基因在永生化猪血管内皮细胞的表达
Genomic Expression Profiling of Peripheral Blood Leukocytes of Pigs Infected with Highly Virulent Classical Swine Fever Virus Strain Shimen
猪瘟病毒感染猪外周血白细胞转录组变化的研究
Determination of Sensitivite Age of Chinchilla Rabbits on Rabbit Viral Hemorrhagic Disease Virus
青紫蓝兔对兔瘟病毒易感日龄的测定
Immunological Analysis of the E2 Glycoprotein of Classical Swine Fever Virus in Rabbits
猪瘟重组蛋白E2的兔体免疫保护试验
Preparation and Identification of Hyperimmune Rabbit-anti-CSFV Serum
兔抗猪瘟高免血清的制备及纯度鉴定
Preparation of Monoclonal Antibodies Against CSFV and the Immunohistochemistry Identification;
猪瘟病毒单克隆抗体的制备及其免疫组织化学鉴定