Molecular Cloning and Expression of Tryptophanase Gene of Escherichia coli;
大肠杆菌色氨酸酶基因的克隆与表达
The gene encoding BspTI site-linker peptide(Gly-Ser-Gly-Ser-Gly-6His-Tag-Eksite(Asp-Asp-Asp-Asp-Lys) was synthesized according to the preferential codons of Escherichia coli,and tryptophanase gene were spliced by overlap extension.
按照大肠杆菌偏爱密码子合成编码"BspTI位点-连接肽(Gly-Ser-Gly-Ser-Gly-6His-Tag-Ek-site(Asp-Asp-Asp-Asp-Lys)"序列的DNA序列,利用重叠延伸拼接技术,将其与色氨酸酶基因TnaA拼接,利用BspTI和HindⅢ重组至pET-39二硫键形成蛋白DsbA基因的下游,转化表达宿主Escherichia coliBL21(DE3),成功构建分泌融合表达型色氨酸酶基因工程菌。
The activities of tryptophanase from eight genetic engineer.
将合成的ＳＯＰＣ应用于８ 株工程菌和１ 株宿主菌的色氨酸酶活力测定，结果表明工程菌色氨酸酶的活力比宿主菌均有不同程度的提高，其中ＷＷ－５３ 号比宿主菌高１５ 倍。
Breeding of tryptophanase-producing Escherichia coli by use of N~+ ion beam implantation
低能N~+注入产色氨酸酶大肠杆菌的育种研究
A Study on Expression Changes of Tryptophan Hydroxylase and Tyrosine Hydroxylase after Multiple Cerebral Concussion in Rats;
三重脑震荡鼠脑色氨酸羟化酶及酪氨酸羟化酶变化研究
Inhibitory Effect of Ginkgolic Acids GA_1 on Mushroom Tyrosinase and B-16 Cell
银杏酸GA_1对酪氨酸酶和黑色素瘤细胞的作用
amino acid RNA ligase
氨基酸rna连接酶
Cloning of Phosphoserine Aminotransferase Gene of Escherichia Coli and Expression in Brevibacterium Flavum;
大肠杆菌磷酸丝氨酸转氨酶基因的克隆和在黄色短杆菌中的表达
aspartic transaminase
天冬氨酸氨基移转酶
ornithine carbamyl transferase
鸟氨酸氨基甲酰转移酶
cDNA Cloning of Rice Blast Inducible Rice Tryptophan Decarboxylase Gene;
稻瘟菌诱导性水稻色氨酸脱羧酶基因的cDNA克隆
Effect of Extracts from Bamboo Leaves on the Tyrosinase and Melanoma Cells
竹叶提取物对酪氨酸酶和黑色素瘤细胞的影响
ornithine decarboxylase
鸟氨酸脱羧酶 鸟氨酸脱羧酶
Deamination of threonine by the enzyme threonine deaminase produces α-ketobutyrate.
苏氨酸被苏氨酸脱氨酶脱氨产生α-酮丁酸。
The essential amino acids are leucine, isoleucine, lysine, methionine, phenylalanine, threonine, tryptophan, and valine.
必需氨基酸包括亮氨酸、异亮氨酸、赖氨酸、蛋氨酸、苯丙氨酸、苏氨酸、色氨酸和缬氨酸。
phosphoribosyl pyrophosphate aminotransferase
磷酸核糖焦磷酸转氨酶
Purification and Characterization of an ε-Poly-L-lysine-degrading Enzyme Isolated from Streptomyces diastatochromogenes TUST2
淀粉酶产色链霉菌TUST2中ε-聚赖氨酸降解酶的纯化和性质
Analysis of Des-Ala-aprotinin and Des-Ala-des-Gly-aprotinin in Aprotinin by Capillary Zone Electrophoresis
CZE分析抑肽酶中去丙氨酸-抑肽酶和去丙氨酸-去甘氨酸-抑肽酶
glutamate oxaloacetate transaminase
谷氨酸 草酰醋酸氨基移转酶
The keti acid is converted to isoleucine by the activity of amino transferases.
酮酸被氨基转移酶转变为异亮氨酸。
methionine adenosyltransferase
甲硫氨酸腺苷转移酶