Meanwhile, whole cell 0 antigen-ELISA, bacterialagglutination test and hemagglutination inhibition assay demonstrated that LPS-O antigen could be expressed on the cell surface by E.
经GM1-ELISA分析表明该菌株能够表达特异的霍乱CT-B抗原,且能分泌到胞外,通过菌体凝集,全细胞O抗原酶联分析和血凝抑制试验表明在1046(pMG305)菌体表面表达了霍乱的O抗原,它的脂多糖O抗原通过SDS-PAGE电泳分析,显示它表达了霍乱LPS的特征区带。
The thin layer chromatography results of culture extract indicated that toxin may be a reductive polysacchride.
病原菌分泌的毒素是一种还原性多糖 ,是致病的主要成分 ,在 2 0℃～ 2 5℃、振荡、连续黑暗和 p H6条件下培养 1 2天产毒最多。