To explore the effect of C-terminal region residues on the molecular conformation and enzymatic activity of a novel cyclic imide hydrolase (CIH), several mutants of CIH, truncated or substituted at C-terminal residues, were designed on the basis of its gene sequence.
为了研究一个新环酰亚胺水解酶(CIH)C端区残基对酶分子构象及酶活性的影响,设计了C末端缺失1~4个氨基酸残基以及C-末端2个Lys替代为2个Glu或2个Leu的突变酶,以野生型酶基因重组质粒pE-cih293为模板,在相应引物存在下,通过PCR扩增获得突变的CIH基因片段。
Cyclic imide hydrolase (CIH, EC.
环酰亚胺水解酶(Cyclic imide hydrolase，CIH，EC。
Based on the difference in charge and hydrophobicity between amidating moiety and the carboxyl group in a given buffer solution, a new method to analyse the Cterminal amidating structure and the amidating enzyme activity by using capillary electrophoresis was established.
利用酰胺化基团与羧基在一定的缓冲液中表现出不同的带电性和疏水性，建立了以毛细管电泳为基础的多肽Ｃ端酰胺化结构分析和酰胺化酶活性测定的新方法。
Molecular Conformation and Substrate Specificity of Mutant Imide Hydrolases;
突变型环酰亚胺水解酶的分子构象与底物专一性
Gene Cloning, Fusion Expression and Biochemical Properties of a Cyclic Imide Hydrolase;
环亚酰胺水解酶的基因克隆、融合表达和生化性质
ESBLs that is a large species of β -lactamases can hydrolyze the third generation cephalosporins and monobactams.
ESBLs是能够水解第三代头孢菌素和单环酰胺类抗生素的β-内酰胺酶。
Immobilization of N-carbamyl-D-amino acid amidohydrolase
N-氨甲酰基-D-氨基酸酰胺水解酶的固定化工艺
The Finding of Acetylpolyamine Aminohydrolase in Streptomyces Griseus;
产素后灰色链霉菌中发现乙酰多胺氨基水解酶
Study on preparation of glutamine--bioactive peptides by two enzyme-hydrolyzed wheat protein
双酶分步水解法制备谷氨酰胺活性肽的研究
Studies on Immobilized L-asparaginase Based on Polyacrylamide/N,N'-methylenebisacrylamide;
聚丙烯酰胺/N,N'-亚甲基双丙烯酰胺固定化L-天门冬酰胺酶的研究
Identification of Compounds Targeting MMP-2 and MMP-9 from Galloyl Cyclic-imide Derivatives and the Activity of 4i to Inhibit Tumor Invasion and Metastasis;
环酰亚胺类肽化合物对明胶酶活性作用及4i抗肿瘤转移作用研究
Design, Synthesis and Anticancer Activity Study of Cyclic-imide Peptidomimetics as Metalloproteinase Inhibitors;
环酰亚胺类肽金属蛋白酶抑制剂的设计、合成及其抗肿瘤活性研究
Study of OXA Extended Spectrum β-lactamases(ESBLs);
超广谱β-内酰胺酶OXA亚型的研究
Synthesis of pyridine-containing copolyimide via chemical imidization method
化学亚胺化合成含吡啶环共聚聚酰亚胺
polyamide-imide resi
聚酰胺-酰亚胺树脂
Study on Structure and Properties of Nano Organic Aluminous Hydrolysate Hybrid Polyimide Film;
纳米铝盐水解物杂化聚酰亚胺薄膜的结构与性能研究
Study on the Epoxy Resin Adhesive Modified by Polyimide
聚酰亚胺改性环氧树脂胶粘剂的研究
biotinylated phosphoramidite
生物素酰亚磷酰胺，生物素化亚磷酰胺
Research on Hydrolysis Reaction of Nitriles and Amides in Near-Critical Water
近临界水中腈、酰胺的水解反应研究
Study on Preparing Polyimide Fibers via Partly Imidized Process of Poly(Amic Acid);
聚酰胺酸部分成环合成法制备聚酰亚胺纤维的研究
Synthesize and Study on the β-lactam Derivatives of Which Contain 3-(2-butoxyl Group) Succinyl Imine-group Groups;
3-（2-叔丁氧基）丁二酰亚胺基单环β-内酰胺衍生物的合成与研究