External symptoms were observed and compared in Pinus thunbergii inoculated with different inoculants including cell free filtration of the liquid culture of the pathogenic bacterium,Pseudomonas fluorescens GcM5-1A strain,carried by Pine Wood Nematode(PWN),Bursaphelenchus xylophilus,the mixture of aseptic B.
将松材线虫携带的致病细菌无细胞滤液、无菌松材线虫和致病细菌混合物、致病细菌鞭毛蛋白无细胞滤液、无菌松材线虫、无菌松材线虫和非致病细菌混合物接种黑松导致的松树萎蔫的病状与野生松材线虫导致松树萎蔫的病状进行观察和对比,结果为:(1)经培养的致病荧光假单孢菌GcM5-1A菌株的无细胞滤液、无菌松材线虫和致病细菌GcM5-1A混合物接种导致的黑松的病状变化趋势与松材线虫致死的黑松病状是一致的;无菌松材线虫、无菌松材线虫和非致病细菌AM2C混合物不能使黑松发病;(2)致病细菌GcM5-1A无细胞滤液和野生松材线虫导致的黑松病状和病程天数无显著差异;(3)接种无菌松材线虫和致病细菌GcM5-1A混合物的黑松病程显著长于接种野生松材线虫的黑松。