To identify whether gene transfected PC12 cells can stably express objective gene or not,we detected α-synuclein-V5 merged protein by cellular immunofluorescence staining,identified mutant gene sequence by cloning SNCA gene of transfected PC12 cells by PCR amplification and sequencing SNCA gene,and measured the expression of α-synuclein of PC12 cells by Western blot.
为构建长期稳定表达人野生型和A53T突变型α-突触核蛋白的PC12细胞株,我们首先制备了慢病毒表达载体,经测序鉴定正确后转染PC12细胞,然后通过细胞的免疫荧光染色检测α-synuclein-V5融合蛋白,PCR法扩增转基因PC12细胞的SNCA片段后进行测序检测突变基因,以及Western blot法检测PC12细胞α-synuclein的表达,从而鉴定转基因细胞能否稳定表达目的基因。
The occurrence frequency of the mutant gene r in the progenies was measured to study the level of heterosis based on fitness.
从适合度之比能够衡量杂种优势强弱的角度研究随机交配群体中突变基因r的频率的逐代演变规律,获得3个不动点,结果表明:r的频率能够从较小增长到一定程度的前提是RR的适合度比Rr的小,揭示了在环境改变的情况下RR群体不需隔离也能演变成rr群体的一个机理。
Aim:To observe how the mutation gene affects life span of inbred strain Yuyi hairless mice.
目的 :通过对豫医无毛小鼠寿命的观察 ,探讨突变基因对无毛小鼠寿命的影响。
To localize the function site of the mutated gene(s) of PGMR into leaf or male organ, a systmatic photoperiodic experiment was conducted.
为了确定光敏不育水稻中突变基因的作用部位，本研究对农垦５８ｓ等５个品种（系）进行了系统的光周期反应特征分析。
ObjectiveTo map the mutation gene of congenital cataract in a four generation pedigree in north of china.
结果三类晶状体蛋白5个侯选突变基因周围的18对微卫星标记位点的LOD值均<0。
Detection of P53 gene mutations in 14 tumor cell lines;
14株肿瘤细胞P53基因突变的检测
Aanlysis on acrR、marOR multi-drug regulated gene mutations in clinical isolates of Shigella;
志贺菌临床分离株耐多药与调控基因突变关系
Relationship Between Mutation of Goosecoid Gene and Microtia;
Goosecoid基因突变与先天性小耳畸形的关系
Effect of the gene mutations associated with knockdown resistance on sodium channel function in pest insects;
昆虫击倒抗性基因突变对钠通道功能的影响
The structure of sodium channels and gene mutations associated with knockdown resistance in insects;
昆虫钠通道的结构和与击倒抗性有关的基因突变
Objective To investigate the lamin A/C gene mutations in Chinese familial dilated cardiomyopathy.
目的 检测中国人家族性扩张型心肌病核纤层蛋白A/C(Lamin A/C)基因突变情况。
ENU-induced Mutagenesis in Mice and Mapping and Cloning Several Mutation Genes;
ENU诱导小鼠突变及对部分突变基因的定位克隆
a disease caused by a dominant mutant gene on an autosome.
由于常染色体突变基因导致的疾
missense suppressor
错义（突变）抑制基因，错义（突变）抑制因子
suppressor sensitive mutant
抑制基因敏感突变型
Mutant Gene "Sparked Art and Culture"
基因突变 文明曙光
ATP7A Genetic Analysis of Menkes Disease;
Menkes病的ATP7A基因突变分析
Construction of Streptococcus mutans comE Mutant Strain Using In-frame Deletion System
框内缺失突变法构建变形链球菌comE基因突变株
Mutation Analysis of Tumor Suppressor Genes (CDH1 and Ago Gene) Related with Gastric Carcinoma;
胃癌相关抑癌基因CDH1、Ago基因突变分析
constitutive mutant
组成性突变[使基因得到组成型表达的突变]
Screening Gene Mutations of XRCC4 by Denaturing High-performance Liquid Chromatography
变性高效液相色谱法筛选XRCC4基因突变
Both frameshift and missense mutations can be suppressed by intragenic suppressors.
移码突变和异义突变都能被基因内抑制基因所抑制。
Application of Mutation-sensitive Molecular Switch in the Detection of EGFR Mutations Associated with Lung Cancer;
基因突变敏感性分子开关在肺癌EGFR基因突变检测中的应用研究
Study on OPTN Gene Mutation in Glaucoma Family with MYOC Gene T455K Mutation;
MYOC基因T455K位点突变青光眼家系OPTN基因突变的研究
A30P and A53T Mutations of the SNCA Gene in α-synucleinopathies
α-突触核蛋白病SNCA基因A30P和A53T的突变筛查
Induction mutation of mustard gas at tk locus in L5178Y cells
L5178Y细胞tk基因突变试验评价芥子气致突性
Making full use of mutant mice as research models requires correct characterization of the phenotypes that result from induced or spontaneous genetic mutations.
要充分利用突变小鼠作为研究模式，则需要诱发突变或自发基因突变之正确基因表现型特徵。
Overexpression and Site-directed Mutagenesis of cry1Aa Gene from Bacillus Thuringiensis
苏云金杆菌cry1Aa基因的表达和定点突变
Mutations happen in two ways.
基因突变以两种方式进行，