Site-directed mutagenesis of functional gene fragment producing CTX-M-14-type extended-spectrum β-lactamase;
CTX-M-14型超广谱β-内酰胺酶基因的定点突变研究
Study on site-directed mutagenesis and kinetics of trehalose synthase from Thermobifida fusca;
Thermobifida fusca海藻糖合成酶的定点突变及其动力学性质研究
The Expression of hBmp-4 in the Pichia Pastoris and Its Site-Directed Mutagenesis;
hBmp-4在甲醇酵母中的表达及其定点突变
Using recombinant glucagon expression system,the 21st aspartic acid was changed to be alanine by means of site directed mutagenesis.
在获得重组胰高血糖素基因工程菌基础上 ,利用定点突变技术改造其第 2 1位氨基酸天冬氨酸为丙氨酸 ,并经DNA测序证明胰高血糖素基因发生了点突变 。
Two p 16 mutants, P48L and D74N, were obtained by site directed mutagenesis using two step PCR method.
应用ＰＣＲ体外定点突变技术，构建了ｐ１６－Ｐ４８Ｌ和ｐ１６－Ｄ７４Ｎ突变体。
Human brain derived neurotrophic factor gene was used as template for oligonucleotide based site directed mutagenesis and PCR.
应用寡核苷酸诱导的定点突变和ＰＣＲ技术对人脑源性神经营养因子基因进行突变，并完成了测序鉴定。
A site-directed mutation at 2753 bp of the ret cDNA induced by PCR;
PCR介导的ret基因体外定点突变
Four conservative tryptophan sites were selected to actualize site-directed mutation.
选取了四个色氨酸保守性位点,分别进行定点突变,构建了CpeS的四个色氨酸突变体,分别为CpeS(W14I)、CpeS(W69M)、CpeS(W75S)和CpeS(G119C,W140V)。
OBJECTIVE: To obtain sequence of α—glucosidase, SWISS-MODEL Server predicted that enzyme structure, amino acid property and characteristic, to design molecular of alpha-glucosidase,make two site-directed mutation by TaKaRa MutanBEST Kit,express the mutant gene in E.
目的:使用PCR技术获得α—葡萄糖苷酶基因序列,通过SWISS-MODEL服务器对α—葡萄糖苷酶结构进行预测,结合氨基酸的性质特点,对酶蛋白进行分子设计,采用PCR突变试剂盒对其进行定点突变,并在大肠杆菌中表达,测定了重组菌、突变菌的酶活力,为进一步研究α—葡萄糖苷酶结构与功能的关系奠定了基础。
Therefore, the method of site-specific mutagenesis was used to turn conservative asparagines into hydrophobic .
基于此,采用定点突变技术对该基序中保守的天冬酰胺进行了一系列的疏水性缬氨酸突变和亲水性的谷氨酰胺突变,并将这一系列突变体克隆至原核表达载体pET-11a中,在大肠杆菌BL21(DE3)中进行了高效表达与初步纯化,为后续测定其热滞活性的变化和验证天冬酰胺的重要性打下了基础。
Methods PCR point mutation was induced by mutating primer and gene vector of PDIP1,the product of PCR and vector pGEX-4T-2 were digested by EcoRⅠand SalⅠ,the pGEX-4T-2-PDIP1 was ligased by the objective segments and them.
方法利用定点突变PCR对PDIP1编码序列进行定点突变,然后将PCR定点突变产物和pGEX-4T-2载体经EcoRⅠ与SalⅠ酶切,再利用连接酶将它们定向连接起来形成pGEX-4T-2-PDIP1重组质粒,转化E。
Site-directed Mutagenesis and Random Mutagenesis of Penicillium Expansum Lipase;
扩展青霉脂肪酶的定点突变及随机突变
Overexpression and Site-directed Mutagenesis of cry1Aa Gene from Bacillus Thuringiensis
苏云金杆菌cry1Aa基因的表达和定点突变
Site-Directed Mutagenesis Analyses, Codon Optimization Study of Harpin_x Protein;
Harpin_x蛋白定点突变和密码子优化研究
AN IMPROVEMENT ON SITE-DIRECTED MUTAGENESIS USING PCR
利用PCR进行基因定点突变方法的改进
Point Mutant of OsAMT1.2 by Using Gene Splicing by Overlap Extension
利用重叠PCR技术对OsAMT1.2进行定点突变
Functional Analysis of Trehalose Synthase in Meiothermus ruber CBS-01 by Site-directed Mutation
利用定点突变分析海藻糖合酶的功能
Site-directed Mutagenesis,Expression and Purification of PZR
PZR基因的定点突变、表达和纯化
Rapid site-directed mutagenesis on full-length plasmid DNA by using designed restriction enzyme assisted mutagenesis
用DREAM技术进行全长质粒快速定点突变
Application of Two Methods for Site-specific Mutagenesis of Porcine IκBα
猪IκBα基因定点突变中两种方法的应用
Site-specific Mutagenesis and Identification of F Gene of NA-1 Strain of Goose Paramyxovirus;
定点突变鹅源副粘病毒NA-1株F基因及其鉴定
Molecule-Directed Evolution and Site-Directed Mutagenesis of D-Hydantoinase;
D-海因酶的体外分子定向进化和定点突变
The Site-directed Mutagenesis of hBMP7 and Its Expression and Characterization Using Pichia Pastoris;
hBMP-7的定点突变及其在甲醇酵母中的表达鉴定
Construction of bsh Promoter Mutants by Rapid One-step Site-directed Mutagenesis
一步法定点突变技术快速构建bsh基因突变启动子
Cloning, Site-specific Mutation and Functional Analysis of Chemical-inducible Promoter;
化学诱导启动子的克隆、定点突变及功能分析
Site-direct Mutation and Eukaryotic Expression of the Human Growth Hormone Receptor;
人生长激素受体的定点突变及其真核表达
The Expression Vector Construction and Site-directed Mutation in Vitro of RET Oncogene;
RET基因真核表达载体的构建及其体外定点突变
Site-directed Mutagenesis of Gln49-PLA_2 and Study on Enzymatic Activity;
Gln49-磷脂酶A_2基因定点突变及酶活性研究
The Expression of hBmp-4 in the Pichia Pastoris and Its Site-Directed Mutagenesis;
hBmp-4在甲醇酵母中的表达及其定点突变