Molecular Cloning of Mature Peptide of Lingnan Yellow Chicken Myostatin Gene and Its Expression in Pichia pastoris;
根据Genebank上登录的鸡的myostatin基因cDNA全长序列以及成熟肽序列设计一对引物,并分别在两引物前设计两个酶切位点EcoRⅠ和KpnⅠ,克隆岭南黄鸡肌肉生长抑制激素的成熟肽蛋白编码基因,然后将特异性片段连接到pMD18-T载体,经酶切、PCR鉴定后,构建了岭南黄鸡真核单纯表达载体pPICZαA-MSTN-m,经测序鉴定,结果表明所克隆的myosta-tin成熟肽基因与Genebank上发表的鸡(AF019621)、猪(AY208121)和家鹅(AF440862)的MSTN-m核酸序列同源性分别可达到99%、92%、86%,但翻译后的成熟蛋白氨基酸序列与鸡、猪和家鹅的同源性可以分别达到100%、99。
Effects of frequency and magnetic strength on the growth of Anabaena flos-aquae by pulsed electric magnetic field;
脉冲变频电磁场对水华鱼腥藻生长的效应
Effects of nitrogen and phosphorus limitation on the growth of Chattonella marina;
N、P营养盐对海洋卡盾藻(Chattonella marina)生长的影响
Effect of hydrogen evolution on the growth of nickel dendrite by electrodeposition;
析氢对电沉积镍枝晶生长的影响
Changes of Some Important Aroma Components in Burley Tobacco during Growing, Harvesting and Curing;
生长、采收、调制过程中白肋烟重要香味成分的变化
A State of Growing——Analyzing the Campus of University of Birmingham;
一种生长状态——解读英国伯明翰大学校园
Effects of Nitrogen Forms and Film Uncoveringon Growing,Yield and Quality of Flue-cured Tobacco;
氮肥形态及揭膜对烤烟生长及产量品质的影响
Lactobacillus survive and grow in the thin fermented material;
乳酸菌在稀醪中成活与生长
Effects of the Biological Organic Fertilizer on the Growth and Quality of Tomatoes;
生物有机肥对西红柿生长及品质影响效应初探
Impact of salicylic acid on the growth of two types of bacteria;
水杨酸对两种细菌生长的影响
Effect of Dibutyl Phthalate on Development of Filial Generation Drosophila Melanogaster;
邻苯二甲酸二丁酯对子代果蝇生长发育的影响
Effects of delayed initial feeding on growth and development in jade perch Scortum barcoo larvae;
延迟投饵对高体革鯻仔鱼早期生长发育的影响
Effects of applying potassium fertilizer at different stages on development and leaf quality of flue-cured tobacco;
分期追施钾肥对烤烟生长和品质的影响