The canines were immuned by regulary with Canine distemper virus(CDV),Canine parvovirus virus(CPV),Infectious canine hepatitis virus(ICHV),Canine coronavirus(CCV),Canine parainflu-enza virus(CPIV).
方法:选择1岁以上临床检查健康的昆明狼种犬及部分土犬20条,用犬瘟热病毒(CDV)、犬细小病毒(CPV)、犬传染性肝炎病毒(ICHV)、犬冠状病毒(CCV)、犬副流感病毒(CPIV)弱毒和强毒多次交叉免疫,颈动脉无菌采血,分离血清,实验室采用微量中和试验、血凝抑制试验来检测抗体效价。
The specific transfer factor(TF) is extracted from spleen of canine that have been immunized with attenuated canine distemper vaccine(CDV).
本试验用犬瘟热弱毒苗免疫的犬 ,提取了特异性犬脾转移因子 (TF) 。
Histopathological observation of canine distemper in pet dog;
宠物犬犬瘟热的病理组织学观察
Systemic pathologic lesions and treatment of dogs with natural canine distemper;
犬瘟热的系统病理损害与治疗
Study on Storage Period of Mink Canine Distemper Live Vaccine of Vero Cell Culture;
水貂犬瘟热Vero细胞活疫苗保存期试验研究
Cloning and expression of canine distemper virus H protein gene fragment;
犬瘟热病毒疫苗株H蛋白基因片段的克隆与表达及表达产物抗原性的初步鉴定
Establishment of an indirect ELISA with a recombinant nucleocapsid protein as antigen for detection of antibodies against canine distemper virus;
犬瘟热病毒重组核蛋白间接ELISA方法的建立
Prokaryotic expression of canine distemper virus nucleocapsid protein and development of indirect ELISA for detection of it s antibody;
犬瘟热病毒核蛋白基因的原核表达及其间接ELISA检测方法的建立
A RT-nested PCR assay for detecting canine distemper virus(CDV) was established using 2 pairs of primers.
根据犬瘟热病毒(CDV)弱毒株Onderstepoort的核衣壳蛋白(NP)基因序列设计了套式引物,建立了RT-nested PCR检测方法。
The nucleocapsid protein gene(N)located 108 to 1679 situation of Canine distemper virus(CDV)genome and was immunogenicity protein with strong conservatism,so N gene was choosed as aim gene.
核蛋白基因(N)位于犬瘟热病毒基因组的108—1679位置处,是保守性较强的免疫原性蛋白,因此选择N基因作为目的基因,利用酶切、连接等方法构建了含犬瘟热病毒核蛋白基因的穿梭质粒pVAX?E3LPN。
Cloning and Sequence Analysis of CDV H,F,N Gene;
犬瘟热病毒小熊猫株H、F和N基因的克隆及表达
Expression of CDV F1 Protein in E.coli and Preliminary Use of the Expressed Protein in ELISA;
犬瘟热病毒F1基因的原核表达与初步应用
A SYBR Green I based Real-time RT-PCR assay was developed for detection of canine distemper virus(CDV) using a pair of primers derived from the nucleocapsid protein(NP) gene sequence.
根据GenBank发表的犬瘟热病毒(CDV)核衣壳蛋白(NP)基因序列,设计合成一对引物,建立了基于SYBR GreenⅠReal-time RT-PCR检测CDV核酸。