In order to develop an indirect ELISA for diagnosing bovine paratuberculosis, the DNA fragmentsof map086 2and map2154c were amplified from the genome DNA Mycobacterium avium Subspeciesparatuberculosis K10.
为了建立一种有效的牛副结核病间接ELISA方法，本研究设计了两对引物，从副结核分枝杆菌P_(18)株的基因组中PCR扩增出两个目的基因map0862和map2154c、，采用重叠延伸剪接技术(splice by overlapping extension，SOE)获得融合基因map0862-2154c。
Development of indirect enzyme-linked immunosorbent assay for detecting deer serum antibodies against Mycobacterium avium subsp.paratuberculosis;
鹿副结核病抗体间接ELISA检测方法的建立
Rapid Detection of Paratuberculosis in Cattle and Sheep by ELISA;
应用ELISA快速检测牛羊副结核病
Study of microcomplement fixation test for cattle Paratuberculosis and sheep paratuberculosis;
微量补体结合试验检测牛、羊副结核病的研究
Application of the specific tetra-valent fusion protein of Mycobacterium bovis in the diagnosis for bovine tuberculosis;
牛分枝杆菌特异性四联融合蛋白在牛结核病诊断中的临床应用
Quick detection of bovine tuberculosis by fluorescent quantitative PCR and its preliminary application;
牛结核病荧光定量PCR快速检测方法的建立及初步应用
Development of a nested PCR for rapid detection of bovine tuberculosis;
牛结核病巢式PCR快速检测方法的建立
Development of an Indirect ELISA for Diagnosing of Bovine Paratuberculosis Using a Recombinant Multi-Antigen;
牛副结核病多重组抗原间接ELISA方法的建立及应用
tuberculin tested milk
经结核病检验过的牛乳
Investigation on the Control and Pathological Studies of Cow s Tuberculosis in Xining City;
西宁市控制奶牛结核病调查及奶牛结核病病理学研究
Study on the ELISA Diagnosis of Paratuberculosis in Ovine;
羊副结核病ELISA检测方法的研究
Pathogenic Investigation and Molecular Epidemiological of Bovine Tuberculosis
牛结核病病原学调查及分子流行病学研究
The Application of the Synthesize Techniques of Prevention and Control to Tuberculosis in Cow;
综合防制技术在奶牛结核病中的应用
Problems of Bovine Tuberculosis Quarantine Purification
浅谈奶牛结核病检疫净化中存在问题
Construction and Evaluation of Immune Efficacy of DNA Vaccine from Paratuberculosis
副结核病DNA疫苗的构建及其免疫效果评价
Investigation on Epidemiology and Control of Milk Cow s Tuberculosis in Anding District, Dingxi City;
定西市安定区奶牛结核病流行病学与防制调查
Epidemiological Investigation on Tuberculosis of Dairy Cow in Hanzhong Area;
汉中地区奶牛结核病流行病学调查分析
Pathogenic Investigation of Bovine Tuberculosis and Identification of in Vivo Induced Antigen Genes of M. Tuberculosis;
牛结核病原学调查及结核分枝杆菌体内诱导抗原研究
The Investigation and Prevention and Cure Study of Strategy of Bovine Tuberculosis in the Xinjiang Region;
新疆部分地区牛结核病监测与防治策略研究
The Research about Mass Survey, Prevention and Cure of the Milch Cow Tuberculosis in Dazhou City;
达州市奶牛结核杆菌病普查及防制技术措施
Optimization and Application in Diagnosis of IFN-γ Detection of Bovine Tuberculosis
牛结核病γ-干扰素诊断方法的优化与应用
Establishment and Application of PCR for Detecting Mycobacterium tuberculosis
牛结核杆菌病PCR检测方法的建立及初步应用
To explore the side effect of the short course intermittent chemotherapy which is the regimen of WHO for pulmonary patients.
目的：探索WHO推荐的短程间歇化疗方案，治疗肺结核病人毒副反应。
Investigation and Prevention of Dairy Cattle Tuberculosis and Brucellosis in Changji;
昌吉市奶牛结核病、布鲁氏菌病普查与防治对策研究
Technological Measures to Improving the Accuracy of Detection of Bovine Tuberculosis in Dairy Cows
提高奶牛结核病群体检疫结果准确性的技术措施