Observation of the morphological change during the adipogenesis differentiation of human bone marrow stromal cell;
人骨髓基质干细胞体外诱导成脂过程形态学变化的观察
Effects of Alendronate sodium on osteogenic and adipogenic differentiation of bone marrow stromal cells;
阿伦磷酸钠对小鼠骨髓基质细胞成骨和成脂分化的影响
OBJECTIVE To study the effects of 17 β-estradiol on the osteogenic and adipogenic differentiation of primary mouse bone marrow stromal cells(MSCs)and the adipocytic trans-differentiation of primary mouse osteoblasts(OBs).
目的研究17β-雌二醇对小鼠原代骨髓基质细胞成骨、成脂分化以及小鼠原代成骨细胞成脂横向分化的影响。
ObjectiveFrom the point of cytomorphology and marker protein (LPL, ALP, OCN),it observed the effect of adipogenic differentiation and osteoblastic differentiation of BMSCs on Bushen Therapy, Huatan Therapy, Bushen Huatan Therapy, and explore the mechanism of Bushen Huatan Therapy of treating osteoporosis.
目的:从形态学、标志物蛋白合成(LPL、ALP、OCN)的角度,分别观察补肾法、化痰法、补肾化痰法对BMSCs成脂分化和成骨分化的影响,探讨补肾化痰法治疗骨质疏松症的可能作用机制。
Objective To investigate the effect of ghrelin on adipocytic differentiation of bone marrow stromal cells (BMSCs).
目的观察胃促生长素ghrelin对原代培养小鼠骨髓基质细胞成脂分化的影响。
The purpose of the experiment is to investigate the function of ATRA on the proliferation and adipogenesis differentiation of porcine adipose mesenchymal stem cells(AMSCs).
利用MTT比色检测不同浓度的全反式维甲酸(alltransretinoic acid,ATRA)对猪AMSCs增殖的影响;光学显微镜下观察猪AMSCs向脂肪细胞分化的形态学变化;油红O染色提取法分析不同浓度ATRA对猪AMSCs成脂分化的影响;RT-PCR检测脂肪细胞分化标志基因LPL和aP2mRNA的变化。