An anion-exchange high performance liquid chromatographic (HPLC) method was developed to determine the proportion of plasmid DNA in both opened and supercoiled forms.
采用阴离子交换高效液相色谱法监测重组质粒pUDKH的生产过程和该质粒产物中超螺旋pUDKH的比例。
To detect the effect of different proportions of supercoiled plasmid on transfer efficiency and expression level in target cells.
结果表明,用超螺旋比例分别为85。