Using RT-PCR-RFLP to detect and analysis potato leafroll virus;
马铃薯卷叶病毒RT-PCR-RFLP的检测分析
The cDNA Cloning and Nucleotide Sequence Analysis of Potato Leafroll Virus Intergenic Region;
马铃薯卷叶病毒基因间隔区的克隆及序列分析
One-step reverse-transcription polymerase chain reaction(RT-PCR) protocol for detection of potato virus X,potato virus Y and potato leafroll virus in infected potatoes and tobaccoes was developed.
运用一步法RT-PCR对马铃薯和烟草中的马铃薯X病毒、马铃薯Y病毒和马铃薯卷叶病毒进行了检测,实验表明:对酚-SDS方法提取的病毒核酸进行扩增,一步法RT-PCR检测到的扩增产物的灵敏度较两步法RT-PCR的高100倍左右。
A pair of DNA primers were designed and synthesized based on the nucleotide sequence of potato leafroll virus(PLRV).
根据马铃薯卷叶病毒(PLRV)外壳蛋白基因的保守序列设计了一对寡聚核苷酸引物,从田间自然感染PLRV的马铃薯病株中提取病毒总RNA,用反转录-聚合酶链式反应扩增出符合设计大小240bp的特异性产物,而对照没有任何扩增产物。
Construction of Double-stranded RNA Interference Vector Against Potato Leafroll Virus;
马铃薯卷叶病毒双链RNA干涉载体构建
Study on Detection of PVY and PLRV Using Taqman Real-Time Quantitative PCR
马铃薯Y病毒和马铃薯卷叶病毒的实时荧光同步检测技术的研究
Research on the detection of PVA and PLRV by Duplex RT-PCR
双重RT-PCR同步检测马铃薯A病毒和马铃薯卷叶病毒
The Molecular Identification and Detection Methods of Potatoleafroll Viruse;
马铃薯卷叶病毒的分子鉴定与检测技术
Cloning, Prokaryotic Expression of PLRV CP Gene and Preparation of Antiserum;
马铃薯卷叶病毒CP基因的克隆、原核表达及抗血清的制备
Study on Genetic Diversity of Germplasm Resources in Sugarbeet and the Detection of Ribozyme Gene in Transgene Potato;
甜菜种质资源的遗传多样性研究与马铃薯卷叶病毒核酶的检测
Construction of Expression vector Vector of Deletion Mutation CP Gene of Potato Leafroll Virus for Saccharomyces cerevisiae
马铃薯卷叶病毒缺失突变CP基因酿酒酵母表达载体的构建
Studies on Principles of the Inhibition to Photosynthesis after Potato Virus Y Infection;
马铃薯Y病毒侵染对马铃薯叶片光合抑制机理的研究
mosaic-type degeneration of potato
马铃薯花叶型退化病
Identification of a New Menber of Genus Potyvirus--Thunberg Fritillary Mosaic Virus;
一个侵染浙贝母的马铃薯Y病毒属新成员——浙贝母花叶病毒的鉴定
Molecular Variability and Synergism of Potato Virus X and Tobacco Vein Banding Mosaic Virus;
马铃薯X病毒和烟草脉带花叶病毒的分子变异及协生作用
Molecular Characterization of Turnip Mosaic Virus, Potato Virus Y and Tobacco Vein-banding Mosaic Virus in China;
三种马铃薯Y病毒属病毒的分子特性
In Vitro Tissue Culture and Virus-Free Detection of Potato;
马铃薯组织培养脱毒和病毒检测研究
Epidemic Survey on Potato Virus and Detection and Identification of Potato Virus S in Zhangjiakou City of Hebei Province
马铃薯病毒疫情调查及马铃薯S病毒的检测鉴定
Advance of Virus Detection of Virus-Free Seed(Seedling) Potatoes
马铃薯脱毒种薯(苗)病毒检测技术研究进展
Simultaneous Detection of Multiple Potato Viruses with Multiplex RT-PCR;
马铃薯病毒多重RT-PCR检测技术研究
RNA-Mediated Virus Resistance in Transgenic Potato;
RNA介导的转基因马铃薯抗病毒研究
A Study on the one-step RT-PCR for detection of potato Viruses;
马铃薯病毒一步法RT-PCR诊断研究