In single enzyme RT PCR,Taq enzyme plays as roles of both reverse transcripase and DNA polymerase.
根据Taq酶既有聚合酶活性又有反转录酶活性的特点 ,探索了只在Taq酶单独作用下完成RT PCR反应的条件 ,以达到对马铃薯纺锤块茎类病毒 (PSTV)的快速检测。
Taq DNA polymerase is the first one which is applied in the PCR.
利用Taq酶的热稳定性,将破胞液在75℃水浴1h,然后用硫酸铵沉淀法获得纯化的表达蛋白。
Comparasion two methods of preparing Taq DNA polymerase;
2种制备Taq DNA聚合酶方法的比较
Taq DNA polymerase has the activities of DNA polymerase and RNA reverse transcriptase.
利用Taq DNA聚合酶既具有DNA聚合酶活性义具有反转录酶活性的特点,探索了在Taq DNA聚合酶单独作用下以双链RNA为模板进行PCR反应的条件。
5X 105 units of Taq DNA polymerase, which could be applied i.
5kb的DNA片段,扩增片段重组到pUC18中测序证实为Taq DNA聚合酶基因,将该片段重组到pBV221温控表达质粒中,在大肠杆菌中表达出94kDa的重组蛋白,100ml培养物的细胞产酶为1。
In this research the Taq plus Ⅰ DNA polymerase with satisfying fidelity was selected to study its reverse transcription activity using mouse epidermal growth factor mRNA as template.
选择具有高保真性的Taq plusⅠ DNA聚合酶,以小鼠表皮生长因子mRNA为模板研究其反转录性能。
Optimization of Preparation Technology of Taq DNA Polymerase;
Taq DNA聚合酶制备技术的优化
Modification and Applications of Taq DNA Polymerase;
Taq DNA聚合酶的改造及应用
Study on Site-directed Mutagenesis of Taq DNA Polymerase and Its Fidelity;
Taq DNA聚合酶的定点诱变及保真度研究
Rapid Purification of High-activity Recombinant Taq DNA Polymerase;
快速纯化高活力基因工程Taq DNA聚合酶
Reversion Analysis for Five Mutation Sites Altered Polymerase Function in the Large Fragment of Taq DNA Polymerase;
五个突变位点对大片段Taq DNA聚合酶聚合功能影响的研究
dna dependent rna polymerase
依赖dna的rna聚合酶
Development of a TaqMan fluorescent quantitative RT-PCR assay for detection of porcine IFN-γ mRNA
猪IFN-γ mRNA Taq Man荧光定量RT-PCR检测方法的建立
The Study of DNA Polymerase Binding with DNA by SPR Biosensor;
SPR生物传感器用于DNA聚合酶与DNA结合的研究
Study on the Role of DNA Polymerase κ and DNA Polymerase η in Alkylating Agent MNNG Induced Untargeted Mutagenesis;
DNA聚合酶κ和DNA聚合酶η在烷化剂MNNG引起的非定标突变中的作用
SERUM HBV-DNA Detected with Polymerase Chain Reaction
应用聚合酶链反应(PCR)检测血清HBV-DNA
Human Carcinoma of Esophageal Eca9706 Cell DNA Polymerseβ Gene Targeting;
食管癌细胞Eca9706 DNA聚合酶β基因敲除
Research progress on DNA polymerase β and genetic instability
DNA聚合酶β与遗传不稳定性研究现状
Development and application of a real-time Fluorogenic Taq Man PCR assay for rapid detection of Pseudorabies virus
Taq Man实时荧光定量PCR检测猪伪狂犬病病毒方法的建立与初步应用
Development of Taq Man fluorescence quantitative PCR assay for detection of chicken anemia virus
鸡贫血病毒Taq Man探针荧光定量PCR检测方法的建立
Klenow fragment
[大肠杆菌DNA聚合酶I的]克列诺片段，大片段（酶）
DNA polymerases are capable of editing and error correction, but RNA polymerases do not appear to have this capacity.
DNA聚合酶可以校对和修正，但是RNA聚合酶没有这种能力。
Most mammalian cells contain multiple species of DNA dependent DNA polymerases.
大部分哺乳动物细胞均含有多种依赖DNA的DNA聚合酶。
cycle sequencing
循环测序法[例如用嗜热DNA聚合酶和热循环仪直接对双链DNA进行测