Cloning of Qβ RNA replicase cDNA gene and its expression in E.coli;
Qβ噬菌体RNA复制酶基因的克隆及在大肠杆菌中的表达
Objective To explore the enhancing effect of RNA replicase of alpha virus on the immune effect of genetic vaccine in order to obtain its optimal form.
目的 探讨α 病毒RNA复制酶对基因疫苗的免疫学效应的加强作用 ,寻找更好的基因疫苗形式。
The RNA replicase gene of foot-and-mouth virus was amplified and cloned into prokaryotic expression vector pET-32a(+).
利用 RT- PCR扩增了口蹄疫病毒 (FMDV )的 RNA复制酶基因 ,并将其克隆到原核表达载体 p ET- 32 a(+)中。
Immunity of genetic vaccine based on alpha virus replicase to human melanoma;
基于α-病毒复制酶的基因疫苗对人黑色素瘤的免疫作用
Research advances in tobacco mosaic virus replicase mediated resistance;
烟草花叶病毒复制酶介导抗性的研究进展
Cloning of GDD deleted replicase gene of Cucumber mosaic virus and construction of its plant expression vector;
缺失GDD保守区的黄瓜花叶病毒复制酶基因的克隆及植物表达载体构建
In researches involving in vitro protein synthesis and self-replication system, Qβ replicase is one of the key enzymes, which are demanded for the high availability.
利用普通表达载体合成Qβ复制酶时 ,得到的 β亚基几乎都是不溶性蛋白 ,从而影响了Qβ复制酶的活性和产率。