0 kb DNA fragment containing the modified enhanced green fluorescent protein CDNAM1 (EGFP S147/P) andSV40 poly(A) signal sequence was amplified and cloned in frame behind the first eight codons of the nonessential glycoprotein G (gG) of pseudorabies virus (PRV) to yield a transfer plasmid.
将绿色荧光蛋白突变体M 1(EGFPS14 7/P)基因融合到伪狂犬病毒 (PRV)非必需糖蛋白 gG的第 8个氨基酸下游 ,通过同源重组、空斑纯化和PCR筛选获得能表达M 1并导致gG基因部分缺失的重组病毒 gG-/M1+ 。
The matured female pupas of Helicovrpa armigera were mutagenized with()~(60)Co γ-rays,a body color mutant was discovered in the hybrid progenies,and the color of the mutant was dark brown.
用60Coγ射线辐照棉铃虫老熟雌蛹,在其后代中发现1只雌性成虫体色突变体,该成虫突变体通体为深褐色。
Genetic Analysis and Gene Mapping on a Rice Albino Mutant XGS;
一份水稻白化突变体的遗传分析及其基因定位
Isolation and Map-based Cloning of Albino Mutant Ems15 in Arabidopsis Thaliana
拟南芥白化突变体ems15的筛选和基因定位
Purification of Human Mutant CD59 Protein and Preparation of Monoclonal Antibody;
人突变CD59蛋白的提取纯化与单克隆抗体的制备
Prokaryotic expression, purification and identification of human mutant nm23-H1 protein
人重组蛋白nm23-H1突变体原核表达纯化与鉴定
Construction of N-linked glycosylation site mutants of envelope glycoprotein genes of hantavirus
汉坦病毒包膜糖蛋白糖基化位点突变体的构建
Purification and Purity Identification of Recombinant Protein rh(GLP-1_(A2G))_2-HSA;
重组人胰高糖素样肽-1突变体的串联体与人血清白蛋白融合蛋白的纯化及纯度鉴定
The Physiological Character and Gene Mapping in a New Green-Revertible Albino Rice Mutant;
一个新的水稻白化转绿突变体的生理特性及基因定位
Purification and Functional Analysis of Nisin Resistance Protein (NSR) Mutation Expressed in Escherichia Coli
乳链菌肽抗性蛋白（NSR）突变体在大肠杆菌中的表达纯化及功能研究
The Influence of α-Synuclein Overexpression on Mitochondrial Membrane Structure with Atomic Force Microscopy
原子力显微镜检测过表达α-突触核蛋白引起的线粒体结构变化
Expression in E. coli of Micrococcus luteus Rpf domain mutants and its purification
复苏促进因子Rpf结构域突变体蛋白的原核表达和纯化
Construction and Functional Study of Human Cubilin Protein Mutants;
人cubilin蛋白突变体的构建与功能研究
Proteome Comparative Analysis of the Seedling of Cry1Cry2 Mutant and Columbia Wild-type-4 of Arabidopsis Thaliana;
拟南芥蓝光双突变体蛋白质组学分析
Studies on Identifying and Tetraploid Inducing in Bud Mutation of White Banana Grape;
白香蕉葡萄芽变突变体鉴定及四倍体诱导研究
Site-Directed Mutagenesis Analyses, Codon Optimization Study of Harpin_x Protein;
Harpin_x蛋白定点突变和密码子优化研究
Purification, Characterization and Crystal Growth of MoFe Protein and HBP59 from a NifE-deletion Strain DJ35 of Azotobacter Vinelandii;
棕色固氮菌突变株DJ35中钼铁蛋白和HBP59蛋白的研究—纯化、特性及晶体生长
Purification, Characterization, Crystal Growth and Identification of Nitrogenase MoFe Protein from Mutant DJ35 and Bacterioferritin from UW3 of Azotobacter Vinelandii;
固氮菌突变种DJ35钼铁蛋白和UW3细菌铁蛋白的纯化、特性、晶体生长及鉴定
The Mechanism of HCV E2 Protein Promoting Activation and Proliferation of B Lymphocyte and Immunogenic Analysis of E2 Mutant
HCV E2蛋白促进B淋巴细胞活化和增殖的机制及E2蛋白突变体免疫原性分析
Diagnostic value of serum anti-mutated citrullinated vimentin antibody in patients with early rheumatoid arthritis
早期类风湿性关节炎患者血清抗突变型瓜氨酸波形蛋白抗体的水平变化意义