Objective: To study myelodyplastic syndrome(MDS) patient about marrow dyshaematopiesis,blast cell detection rate and the number scope of cell.
目的:观察骨髓增生异常综合征患者骨髓的病态造血情况及原始细胞簇的检出率及组成细胞数范围。
BACKGROUND AND AIM:To study the differentiation of mouse liver epithelial progenitor cells(LEPCs) to cholangiocytes induced by hairy and enhancer of split 1(Hes1)gene in vitro.
1-Hes1,将2种表达载体分别转染肝原始细胞系(LEPCs),应用RT_PCR和Real-timePCR技术检测胆管细胞分子标志物CK19、GGT,胆管上皮细胞相关转录因子HNF6、HNF1β,肝细胞分子标志物GS、BGP和肝卵圆细胞的分子标志物Thy-1的表达,并在荧光显微镜下观察EGFP标记的LEPCs细胞系荧光强度的变化。
Gametophore primordium occurred a.
结果表明:孢子第2天就开始萌发,第6天时其萌发率达90%以上;原丝体系统由绿丝体(chloronema)和轴丝体(caulonema)构成,假根(rhizoides)产生于芽体基部,由轴丝体退化而成;配子枝原始细胞产生于绿丝体分枝的基部或轴丝体上的斜壁细胞;配子枝(game tophore)形成后其上各部位都可形成假根;孢子萌发类型为真藓型(Bryum-type)。
The protonema in the submerged condition,which was of slow growth,great length and a few branches,couldn t produce gametophore primordium even at day 80.
)孢子进行培养实验,结果显示:2种培养条件下孢子萌发情况较为一致,萌发率均达90%以上;沉水培养的原丝体生长缓慢、原丝体较长,其分枝数很少,至80 d时仍未出现配子枝原始细胞;而正常培养的原丝体生长较快,其上分枝多而密,约40 d时出现配子枝原始细胞。