To obtain the fertilized eggs with clear male pronuclei is the key of success .
显微注射法是制备转基因动物的首选方法 ,而原核清晰受精卵的获得是影响显微注射成败的关键。
Objective To investigate the relationship between the first polar body morphology of oocyte and the zygote morphology in pronucleus stage, embryo quality in cleavage stage.
目的 探讨卵细胞第一极体形态与原核期受精卵形态、卵裂期胚胎质量的关系。
By combining with the author’s own research work, the progresses of study on sperm egg identification, acrosomal reaction, cortical reaction, and formation of male and female pronucleus in decapod crustacean are reviewed in the paper.
结合作者近期的研究工作，从精卵识别、顶体反应、皮层反应、雌雄原核的形成和结合等方面综述了近年来十足类甲壳动物受精细胞学方面的研究成果，并展望了这一领域的研究与应用前景。
Methods: All embryos with non-pronucleus (0PN), a single pronucleus (1PN), a number of pronucleus (≥3PN) and 2 pronucleus delaying development in cleavage stage (2PN) were cultured into blastula by the sequential method.
方法:通过囊胚序贯培养法将无原核(0PN)、单个原核(1PN)、多个原核(≥3PN)和卵裂期发育延缓的2原核(2PN)废弃胚胎培养至囊胚期。
Prokaryotic expression and purification of truncated Rab8 recombinant protein and preparation of polyclonal antibody against Rab8;
重组Rab8截短体蛋白的原核表达、纯化和抗体制备
Prokaryotic expression,purification and primary bio-chemical properties research for Tnfrsf11b N-terminal protein of homo sapiens;
人Tnfrsf11b-N端多肽的原核表达、纯化及其初步分析
Prokaryotic expression and identification of recombinant swine hepatitis E virus ORF2 main antigen region;
重组猪戊型肝炎病毒ORF2区主要抗原域的原核表达及鉴定
Cloning and procaryotic expression of the dehydrin dhn4 gene from Tibetan hulless barley;
青稞脱水素基因dhn4的克隆与原核表达
Cloning and Procaryotic Expression of Gene of Retinol-Binding Protein;
维生素A结合蛋白基因的克隆及原核表达
The procaryotic expression vector PKENG-M for GFP: CMV MP was constructed by cloning in pKEN.
采用重组－ＰＣＲ方法，扩增获得绿色荧光蛋白（ＧＦＰ）与黄瓜花叶病毒运动蛋白（ＣＭＶ ＭＰ）融合基因，将其克隆到 ｐＫＥＮ上，构建了该融合基因的原核表达载体 ｐＫＥＮＧ－Ｍ。
Relationship among pronuclear morphology,cleaved-embryo development and chromosomal abnormality;
原核形态、卵裂胚发育与染色体异常关系的研究