Quantitive assay for PCR product of hIL-8 mRNA with Enzyme-linked sandwich hybridization;
微量酶联夹心杂交法定量检测hIL-8 mRNA PCR扩增产物
Enzyme-linked sandwich hybridization in microplate for quantification of human TNF-α mRNA;
微量酶联夹心杂交法定量检测TNF-α mRNA
Objective: To establish a RT-PCR-enzyme-linked sandwich hybridization(RT- PCR-ELSH)method for the identification of RSV subgroups A and B and to analyze the epidemics and distributive situations of RSV subgroups A and B in infants with acute lower respiratory tract infections in the southern area of Zhejiang province.
方法:根据RSVG蛋白基因核苷酸序列设计引物和探针,通过逆转录-聚合酶链反应-酶联夹心杂交法(RT-PCR-ELSH)随机检测了浙南地区连续两年冬春季期间202例急性下呼吸道感染的婴幼儿鼻咽部分泌物中RSV及其亚型的病毒核酸;与病毒分离培养、直接免疫荧光法(DFA)、OligoDetect试剂盒比较探讨其临床实用性。