Method: The cDNA probe of esophageal cancer tissue was labeled with digoxigenin by reverse transcription reaction, and applied in reverse northern blot to initially screen the retrieved differential cDNA fragments.
方法 :提取食管癌组织总RNA ,通过逆转录反应 ,用地高辛标记为cDNA探针。
Expression of the mRNA for intrarenal transforming growth factor-β 1 (TGF-β 1) and renin was measured by reverse transcription-polymerase chain reaction (RT-PCR).
方法 :大鼠灌服CsA及低盐饮食 2 8天 ,用逆转录聚合酶链式反应 (RT PCR)的方法观察肾内转化生长因子 β1(TGF β1)、肾素的mRNA表达及TGF β1、Ⅳ型胶原 (CollagenⅣ )免疫组化的改变 ,并观察复方丹参注射液对上述改变的影响。
1.2.3 RT-PCR reaction and agarose gel electrophoresis: take forementioned reversion record reaction product to 25 l reactant intermediate to proceed PCR reaction.
2.3 RI’- PCR反应和琼脂糖凝胶电泳:取上述逆转录反应产物于25川反应体
cyclical transcription and reverse transcription
循环（式）转录（和）逆转录（反应）
reverse transcriptase
【生化】逆转录酶, 反转录酶
Dection of enterovirus infection by reverse transcription and polymerase chain reaction
应用逆转录聚合酶链反应检测肠道病毒感染
Establishment of reverse transcription semi-nested polymerase chain reaction to the pathogenic diagnosis of JE patients
逆转录-聚合酶链反应在乙型脑炎诊断中的建立
Expression of the multidrug resistance in human bladder tumors by reverse transcription polymerase chain reaction
应用逆转录聚合酶链反应技术检测膀胱癌多项耐药基因的表达
Analysis of reserved conditions of blood sample for detection of HCV RNA with RT-PCR
逆转录聚合酶链反应检测HCV RNA血标本保存条件分析
Rapid identification of subtypes of respiratory syncytial virus by real-time reverse transcription PCR
实时逆转录聚合酶链反应快速鉴别呼吸道合胞病毒亚型
Comparison Study of Conventional Method and Reverse Transcription Polymerase Chain Reaction on Detection of Flavivirus in Mosquitoes
逆转录聚合酶链反应与常规法检测蚊体内黄病毒的比较研究
Detection of Rice Stripe Virus by Reverse Transcription and Polymerase Chain Reaction
逆转录聚合酶链式反应快速检测水稻条叶枯病毒
The detection of circulating hepatocellular carcinoma cells in peripheral venous blood by reverse transcription polymerase chain reaction and its clinical significance
巢式逆转录多聚酶链反应检测循环性肝癌细胞及其临床意义
DETECTION OF ENTEROVIRUS RNA IN HUMAN BLOOD SPECIMENS BY REVERSETRANSCRIPION POLYMERASE CHAIN REACTION
逆转录聚合酶链式反应检测人血标本中肠道病毒核糖核酸
The Detection of Carcinoma Cells in the Peritoneal Washings of the Patients with Gastric Carcinoma by Nested Reverse Transcription-polymerase Chain Reaction and Its Clinical Significance;
巢式逆转录酶链反应检测腹腔胃癌细胞及其临床意义
Isolation and Functional Identification of Transcripiton Factors That Regulate the Stress Responses in Soybean;
调控大豆抗逆反应的转录因子基因的分离与功能鉴定
Research of Real-time Quantitative Reverse-transcriptase Polymerase Chain Reaction (RQ-PCR) to Detect bcr/abl Fusion Gene in Chronic Myeloid Leukemia;
实时定量逆转录聚合酶链反应检测慢粒bcr/abl融合基因的研究
The Construction of Chicken Oviduct Specific Retroviral Expression Vector of Porcine β-interferon and Production of Transgenic Chicken;
逆转录病毒表达载体的构建及其鸡输卵管生物反应器的制作
The Pre-Exploration of Establishing Chicken Oviduct Bio-Reactor by Retrovirus;
逆转录病毒法制备鸡输卵管生物反应器的前期探索
Detection of mRNA in kidney of dead rat using fluorescence semi-quantitative RT-PCR;
荧光半定量逆转录-聚合酶链式反应检测大鼠死后肾mRNA