The gene fragments HNa, HNb and HNa-L-b coding for the antigenic structural domains of the newcastle disease virus (NDV) hemagglutinin-neuraminidase were amplified from the recombinant plasmid containing full length NDV HN gene by regular PCR or overlap extension PCR with specific primers.
以本实验室构建的含新城疫病毒血凝素-神经氨酸酶基因重组质粒为模板,设计引物通过PCR和重叠-延伸PCR扩增,获得HNa、HNb和HNa-L-b三种抗原结构域基因片段,BamHⅠ/HindⅢ双酶切定向克隆到原核表达载体pET32c,获得重组质粒分别命名为pET32c-HNa、pET32c-HNb和pET32c-HNa-L-b。
Construction and identification of the recombinant adenovirus expressing hemagglutinin-neuramidinase of Newcastle disease virus
含新城疫病毒血凝素-神经氨酸酶基因重组腺病素的构建及鉴定
Cloning and Expression of Hemagglutinin-neuraminidase Gene from Newcastle Disease Virus B95 Strain;
新城疫病毒B_(95)株血凝素—神经氨酸酶基因的克隆与表达
Cloning the Hemagglutinin-Neuraminidase Gene of Newcastle Disease Virus and Expressing It s Main Antigen Region in Prokaryotic Cell;
新城疫病毒血凝素—神经氨酸酶基因的克隆及其主要抗原域的原核表达
Mimotope of Hemagglutinin-neuraminidase from Newcastle Disease Virus and Its Immunological Properties;
新城疫病毒血凝素—神经氨酸酶模拟表位及其免疫生物学特性研究
Purification of H5N1 Hemagglutinin Expressed by Recombinant Vaccinia Viruses and Kinetics of Anti-Oseltamivir Neuraminidase
H5N1血凝素蛋白的重组牛痘病毒表达纯化以及耐奥司他韦神经氨酸酶动力学研究
After the treatment with neuraminidase, the distributions of WGA receptors changed apparently.
用神经氨酸苷酶处理后,麦胚凝集素受体的分布有明显变化。
Study on Correlativity of Gene Expression between Tyrosine-kinase Receptors and Vascular Endothelial Growth Factor in Human Neuroblastomas
神经母细胞瘤中酪氨酸激酶受体和血管内皮生长因子基因表达相关性的研究
The Influence Factor on ALT Test for Donated Blood in Baotou City
影响包头市献血员丙氨酸氨基转移酶测定结果因素的分析
Hemogglutinin Gene and Amino Acid Characteristic of Measles Virus in Jilin Province,2001-2008
吉林省2001~2008年麻疹病毒血凝素蛋白基因特征和氨基酸变异分析
The effect of time factor on differentiation of NSCs to GABA neurons
时间因素对神经干细胞向γ-氨基丁酸能神经元分化的影响
Study on Neuraminidase Gene s Molecular Evolution of Human Influenza Virus (H3N2) Isolated in China;
中国H3N2亚型人流感病毒神经氨酸酶基因的分子进化研究
Genetic characterization of neuraminidase gene of novel influenza virus A/H1N1 in 2009 pandemic
2009年新型甲型H1N1流感病毒神经氨酸酶基因进化分析
The Association of Protein Tyrosine Phosphatase 1B Gene and Leptin Receptor Gene Polymorphisms with Hypertension with Insulin Resistance;
蛋白酪氨酸磷酸酶-1B基因和瘦素受体基因多态性与高血压胰岛素抵抗相关性研究
③ Amino acid analysis of ACE inhibitory peptides.
③血管紧张素转换酶抑制肽的氨基酸分析。
Subunit separation and sequencing of agacutin from agkistrodon acutus snake venom
尖吻蝮蛇蛇毒类凝血酶Agacutin的亚基的拆分和氨基酸残基测序
Prokaryotic expression of serpin-2 gene from Haemaphysalis longicornis
长角血蜱丝氨酸蛋白酶抑制剂基因的原核表达
Study on Amino Acids in Striatum and Blood Pressure;
纹状体内氨基酸类神经递质与血压的关系研究
Detection of the Antibodies Level of Swine Influenza Virus、Neuraminidasecloning and Initial Construction of Prokaryotic Expressing Vector;
猪流感病毒抗体水平监测、神经氨酸酶基因的克隆与原核表达载体初步的构建