[Methods] The rAAV-GFP,rAAV-LyoVec and rAAV-GFP-LyoVec were Constructed,the transfection efficiency in rAAV-GFP,LyoVec-GFP and rAAV-GFP-LyoVec to quantitative rat C6 cells were observed and measured by fluorescence microscope,and the transfection efficiency in the three vectors were confirmed and compared.
目的探讨重组腺病毒相关病毒(rAAV)-阳离子脂质体(LyoVec)复合载体的构建方法及其转染率评价。