9706 which luciferase expression was regulated by HCV IRES.
脂质体转染法将其导入HCVIRES介导萤光素酶表达的转基因细胞 (HepG2 。
Methods:Human hepatocellular carcinoma cell line HepG2 cells were transduced with vector pCI-neo-Luc encoding luciferase gene.
方法:将包含有萤火虫萤光素酶基因的pCI-neo-Luc载体转染人肝癌HepG2细胞系,筛选获得具有高萤光素酶活性的细胞克隆;利用流式细胞仪对萤光素酶表达的稳定性进行初步研究,并分析细胞的生物发光情况;持续表达萤光素酶的肿瘤细胞培养扩增后被植入裸鼠皮下,2周后以形成的异体瘤作为供体瘤,进行肝脏原位移植手术;对建立的肝癌原位移植模型,用影像学资料显示肿瘤部位,用IVIS成像系统动态监测肿瘤生长情况。
The starch-binding domain (SBD)-encoding region of cyclodextrin glycosyltransferase from Bacillus circulans is fused to the 5′-terminal end of the luciferase (LUC) gene, via an artificial Pro-Thr encoding linker sequence.
将环状糊精糖基转移酶的SBD基因编码序列连接到萤光素酶基因的 5′ 端(SBD LUC)后,重组基因通过农杆菌介导导入马铃薯植株中。
ATP-Bioluminescence method allowed the rapid quantification of microorganisms by measuring their intracellular ATP in the firefly luciferin-luciferase BL reaction.
自20世纪40年代末期虫萤光素-萤光素酶体系在萤火虫中被发现并被用于检测ATP含量，该方法已逐渐成为精确测量ATP水平及检验微生物的快速方法。
Immobilization of firefly luciferase and construction of luciferase biosensor;
虫萤光素酶固定化及其传感器
Construction of a luciferase reporter vector with resistance of neomycin
带新霉素抗性的萤光素酶报告载体的构建
An efficient method for screening effective siRNAs using dual-luciferase reporter assay system
利用双萤光素酶报告基因系统筛选有效的siRNA
Construction and identification of a luciferase reporter vector containing human CXCR4 promoter
人CXCR4基因启动子萤光素酶报告载体的构建及鉴定
Molecular cloning and constructing luciferase report gene vector for the gene promoter of CCDC72,a novel modulator for hair follicule developement
调控毛囊生长CCDC72基因启动子克隆及萤光素酶报告基因载体的构建
Comparison of TTR and CMV Promoters in vivo and in vitro via a Secreted Luciferase Reporter System
用分泌型萤光素酶报告系统比较TTR启动子与CMV启动子的体内外表达特性
A Study on the Properties and Applications of Firefly Luciferase;
萤火虫荧光素酶的性质和应用的研究
Construction of Recombinant Retroviral Vector Containing Firefly Luciferase Reporter Gene
萤火虫荧光素酶报告基因重组逆转录病毒载体的构建及鉴定
Applications of Luciferases in Studies of Plant Promoters and Plant Stress Tolerance;
利用萤光酶研究植物启动子和植物抗逆性
luminescence resulting from a chemical reaction as the oxidation of luciferin in fireflies.
由化学反应发出的光，比如萤火虫的虫萤光素发生氧化反应时发出的光。
Determination of hypoxanthine and xanthine in fish by the coupled reaction of enzyme (fluorometric method)
酶偶联反应测定鱼中的次黄嘌呤和黄嘌呤(萤光光度法)
They added a small amount of the fluorescent dye coumarin to the emitter material tris(8- hydroxy-quinoline) aluminum.
他们在发光材料三（–基口喹啉）铝里，加入了少量的萤光染料香豆素。
Fast atom bombardment mass spectrometry of tetrachlorotetraiodofluorescein and its disodium salt
四氯四碘萤光素及其二钠盐的快原子轰击质谱分析
Fluorescent lamp:Type of electric discharge lamp consisting of a glass tube filled with a mixture of argon and mercury vapor.
萤光灯:藉磷光粉涂层所发萤光照明的放电灯。
xenon automatic fluorescence colour meter
氙气自动萤光测色计
Fireflies emit light but not heat.
萤火虫发光但不放出热。
The activation mechanism of La( III) to the HRP was also discussed.
导致萤光发色基团所处的微环境也相应变化；并运用它推测了镧离子对该酶的激活作用机制。
They added a small amount of the fluorescent dye coumarin to the emitter material tris (8-hydroxy-quinoline) aluminum.
他们在发光材料三（8?羟基口喹啉）化铝里，加入了少量的萤光染料香豆素。
An enzyme present in the cells of bioluminescent organisms that catalyzes the oxidation of luciferin.
荧光素酶存在于生物性发光组织的细胞中，催化荧光素氧化的酶