Preliminary studies have been made on the effect of bound water on the binding of estrogen receptor with estrogen responsive element in vitro.
本文以雌激素受体（ＥＲ）同３２ｂｐ雌激素效应元件（ＥＲＥ）体外结合为模型，对结合水分子的参与进行探索性研究。
[Methods] The oligonucleotides of the mouse mammary tumor viruslong terminal repeat (MMTV-LTR) containing androgen response element (ARE) and promoter were cloned into the kpn Ⅰ and Bgl Ⅱ sites of pCAT3-basic, creating pMMTV-CAT.
[方法]合成含雄激素效应元件(ARE)和启动子的小鼠乳腺癌病毒长末端重复序列(MMTV-LTR),定向插入报告基因质粒pCAT3-basic的Kpn Ⅰ和Bgl Ⅱ之间,构建受ARE调控的CAT报告基因质粒pMMTV-CAT,将表达AR的质粒AR/pcDNA3。
It was shown that the translational product could bind to the estrogen response element(ERE).
利用体外翻译系统，翻译了能与雌激素效应元件（ＥＲＥ）结合的全长的人雌激素受体（ｈＥＲ）。