Objective To construct the prokaryotic expression plasmid expressing woodchuck hepatitis virus core antigen(WHcAg) and prepare polyclonal antibodies.
目的构建土拨鼠肝炎病毒核心蛋白质粒并进行原核表达、抗体制备。
【Method】 The woodchuck hepatitis virus posttranscriptional regulatory element(WPRE) was amplified by polymerase chain reaction(PCR)from the plasmid containing WPRE.
【方法】将PCR扩增土拨鼠肝炎病毒(Woodchuck Hepatitis Virus,WHV)转录后调控元件(WPRE),克隆于pGM-T载体获得重组质粒pGM-WPRE,利用NotⅠ酶将WPRE亚克隆入pcPPTPRE(+),筛选WPRE正向连接的重组质粒pcPPTWPRE,采用磷酸钙法分别将pcPPTWPRE和pcPPTPRE(+)转染HEK293细胞和DF-1细胞,利用荧光显微镜观察EGFP蛋白的表达,利用流式细胞仪检测转染细胞中EGFP阳性细胞的表达率。
hepatitis B surface antigen radioimmunoassay
乙型肝炎表面抗原放射免疫测定
Prokaryotic and Eukaryoyic Expression of the Shortened Hepatitis B Surface Antigen and Their Antigenic Analysis;
截短的乙型肝炎表面抗原分子的表达及其抗原特性的研究
Human Antibody Library Construction by Phage Display;
人源性抗乙型肝炎表面抗原（抗HBs）噬菌体单链抗体库的构建
The Affinity of Beta 2-Glycoprotein I Binding to Hepatitis B Surface Antigen
β2糖蛋白I与乙型肝炎表面抗原亲合力的研究
Analysis of HBsAg Examination Results of 72105 Employees
从业人员72105名乙型肝炎表面抗原检测结果分析
Intra Muscular Injection of the Gene Encoding Hepatitis SurfaceAntigen Induced HBsAg Specific Immune Response in Mice
骨骼肌注射乙型肝炎表面抗原基因在小鼠体内诱发乙肝表面抗原特异的免疫反应
A Comparative Study of Methods Detecting HB Surface Antigen in Liver Biopsy Specimens
肝活检标本中检测乙型肝炎表面抗原方法的比较
LPS_p Increases Production of Anti-HBS in Mice Induced by Hepatitis B Surface Antigen;
LPS_p对乙型肝炎表面抗原诱导小鼠抗体产生的影响
The clinical analysis of both positive mode of HBsAg and HBsAb
乙型肝炎表面抗原及抗体同时阳性少见模式临床分析
Cloning and Expression of Mutant Hepatitis B Surface Antigen in Pichia Pastoris and Their Recognition by Commercial Detection Assay;
变异乙型肝炎表面抗原在毕赤酵母中的克隆表达及表面抗原诊断试剂的评价
Construction of Recombinant CHO Cell Strain for High Expression of HBsAg
高效表达乙型肝炎表面抗原的CHO工程细胞株的构建
Influences of Hepatitis B Virus Genotype on Serum Levels of Hepatitis B Surface Antigen and Its Kinetics under Antiviral Therapies
基因型对血清乙型肝炎表面抗原水平及动态变化的影响
Virological Characteristics of Inactive Carriers of Hepatitis B Surface Antigen
非活动性乙型肝炎表面抗原携带者的病毒学特征
Induction of Cellular Immune Response by HBsAg Combined with rmIL-12 in Mice
乙型肝炎表面抗原联合rmIL-12诱导的小鼠细胞免疫应答
Effect of HBsAg mutation at aa118 position on the HBsAg blood screening test
乙型肝炎病毒表面抗原118位氨基酸变异研究
Comparison of two methods in detecting HBsAg
两种方法检测乙型肝炎病毒表面抗原的比较
Serological Epidemiology of HBsAg and Anti-HBs for Children in Bobai County in 2006
2006年博白县部分儿童乙型肝炎病毒表面抗原和抗体现状调查
Expression of the HBsAg Gene in Escherichia colt
乙型肝炎病毒表面抗原基因在大肠杆菌中的表达