First, the basic principle of activation tagging, and the progress of its applicatio.
与传统的T-DNA插入功能缺失突变相比,建立在功能获得突变基础之上的激发标签技术具有独特的优势,主要表现为可以获得功能冗余基因的显性突变体并方便地克隆相关基因。
Activation tagging is a technique to acquire gain of function mutants, and it has particular advantages in isolating and studying genes from gene family, genes controlling hard-to-test traits, and genes involved in QTL, etc.
激发标签技术是建立在功能获得突变基础上的一项突变体构建技术，它在分离和研究基因家族的基因、性状不易检测的基因以及性状由QTL(quantitative trait loci)控制的基因等方面具有独特的优势。
Five digesting methods concluding three digesting acid systems and two heating modes were compared and discussed for the detection of multi-trace element concentrations in the standard material of human hair (GBW07601) by inductively coupled plasma mass spectrometry(ICP-MS),evaluate the accuracy of this method.
本文采用三种不同酸体系和两种加热方式组合成五种消解方法,对人发标准物质(GBW07601)进行消解,以ICP-MS作为分析技术测定其微量元素的含量,并对其方法、结果及影响因素进行了相应的讨论。