Self-cloning strains of industrial brewing yeast were constructed by integrating Saccharomyces cerevisiae genes, γ-glutamylcysteine synthetase gene (GSH1) and copper resistant gene (CUP1) into the locus of alcohol dehydrogenase II (ADH II) gene (ADH2).
采用自克隆技术,破坏啤酒酵母工业菌株YSF31的ADH2基因,在ADH2基因位点插入来源于YSF31的编码γ-谷氨酰半胱氨酸合成酶的GSH1基因和铜抗性筛选标记CUP1基因。
Construction of Self-cloning Industrial Brewing Yeast with High-glutathione Production and Low-ADH Ⅱ Enzyme Activity;
本文利用自克隆技术,构建了一株新型啤酒酵母工程菌,并对其进行了小型发酵试验,检测了重要的产物含量和发酵指标。
A Survey and Outlook of Research in Breeding of Industrial Brewing Yeast by Self-cloning Technique;
自克隆技术在工业啤酒酵母改良中的研究概况