The samples were separated into Saturates, Aromatics and Polar compounds by HPLC system coupling with dual columns (μ Bandapak CN column and μ Poras il silica gel column), refractive index detector, and backflushing procedure.
以键合氰基柱和硅胶柱双柱切换的方式 ,正己烷为流动相 ,示差折光检测器检测 。
0 mL·min~(-1),the detection was carried out with refractive index detector.
0 mL·min~~(-1),用外标法测定,示差折光检测器检测。
Eight organic acids (including oxalic, citric, α-ketoglutaric, L-malic, succinic, L-lactic, fumaric and acetic acid), glucose, and ethanol were separated by refractive index detector (RID).
8mL/min,采用示差折光检测器对8种有机酸(草酸、柠檬酸、α-酮戊二酸、L-苹果酸、丁二酸、L-乳酸、富马酸和乙酸)、葡萄糖及乙醇进行了成功的分离,确定了各物质工作曲线的回归方程、线性范围、相关系数和检出限。