Determination of isopimaric acid in leaves of Platycladus orientalis and its preparations Cebai No.V Capsula and Isopimaric Acid Capsula by HPLC;
HPLC法测定侧柏叶及其制剂侧柏Ⅴ号胶囊和异海松酸胶囊中异海松酸
Rosin type can be determined and distinguished from the content of isopimaric acid and the quantity ratio of pimaric acid/isopimaric acid.
用纯马尾松松脂、纯湿地松松脂、纯思茅松松脂制得松香样品;应用DB-5毛细管柱对松香样品中主要的树脂酸含量进行了气相色谱定量分析;通过测定松香样品中异海松酸GC含量和海松酸/异海松酸GC含量的比值,可以确定并鉴别松香所属种类。
Result: Eight compounds were isolated and identified as isopimaric acid(Ⅰ), β-sitosterol(Ⅱ), 24R, 5α-stigmast-3,6-dione(Ⅲ), larixol(Ⅳ), ferulic acid(Ⅴ), lariciresinol(Ⅵ), secroisolariciresinol(Ⅶ)and isolariciresinol(Ⅷ).
结果:从其醇提物的氯仿部分分离并鉴定了8个化合物,分别为:异海松酸(Ⅰ),β-谷甾醇(Ⅱ),24R-5α-豆甾烷-3,6二酮(Ⅲ),larixol(Ⅳ),阿魏酸(Ⅴ),落叶松脂醇(Ⅵ),开环异落叶松脂醇(Ⅶ),异落叶松脂醇(Ⅷ)。
Bis-oxiranylmethyl ester of acrylic adduct of levopimaric acid (2) was prepared by acrylic adduct of levopimaric acid (1) with epichlorohydrin in the presence of phase-transfer catalyst.
丙烯海松酸 (1)与环氧氯丙烷在弱碱及相转移催化剂存在下反应得到丙烯海松酸二缩水甘油酯 (2 ) ,探索出较佳的反应路线 ,产物分离纯化后对其结构和性能进行了研究。
RESULTS Five monomers and two mixtures were isolated and identified as pimaradiene, n-docosyl ferulate, n-tetracosyl ferulate, stigmasterol, n-triacontyl cis-p-coumarate, the mixture of n-tetracosyl trans-p- coumarate, n- hexacosyl trans-p-coumarate, n-octacosyl trans-p-coumarate and defuscin, .
分别为海松二烯 ,3 甲氧基 4 羟基反式肉桂酸二十二烷酯 ,3 甲氧基 4 羟基反式肉桂酸二十四烷酯 ,豆甾醇 ,对羟基顺式肉桂酸三十烷酯 ,对羟基反式肉桂酸直链烷酯的4个系列化合物的混合物 ,直链烷醇的 5个系列化合物的混合物。