METHOD: PBMCs were cultured in stem cell growth medium (SCGM) supplemented with monoclonal anti-CD3 antibodies and IL-2 with different concentrations; The immune phenotypes of expanded cells were analyzed by Flow cytometry (ECM).
方法 使用干细胞生长培养基,在抗CD3单抗和IL-2的作用下,从健康成人PBMC中诱导扩增CD3~- CD56~+NK细胞,并用流式细胞仪检测扩增细胞的免疫表型。
Objective Effects of CD3 McAb,CD28 McAb,PHA and lowdose γray irradiation on T lymphocytes were investigated to explore factors of influencing T cell signals transduction.
目的 观察CD3单抗、CD2 8单抗、PHA和低剂量辐射对淋巴细胞增殖的影响 ,探讨影响T淋巴细胞信号转导的因素。
To study genes involved in human T cell signal transduction, a suppression subtractive hybridization (SSH) technique was developed to isolate the cDNA clones of genes that contained specific (differentially expressed) transcrips of T cells activated with anti-CD3 and anti-CD28 serving as“tester”, and the no.
为了研究参与人T细胞信号转导的基因,本文以正常人初始T细胞作为对照组,以抗人CD3单抗联合抗人CD28单抗激发的人T细胞作为实验组,进行抑制性削减杂交。
Immobile CD 3McAb was more potent on activating T lymphocytes than soluble CD 3McAb.
结论：粘附状态抗CD3单抗比游离状态抗CD3单抗具有更强的诱导人T淋巴细胞活化增殖能力。
Experimental Studies on the Killer Cells Co-Induced by the Soluble Head and Neck Tumor Antigens Plus AntiCD3mAb and Interleukin-2;
可溶性头颈肿瘤抗原和抗CD3单抗及白细胞介素2共同诱导杀瘤细胞的实验研究
Molecular Directed Evolution of Reshaped Anti-CD3 Single Chain Fv Fragment;
抗CD3改型单链抗体的分子定向进化
Study on Modification of Monoclonal Antibody Anti-CD3 with mPEG;
PEG修饰鼠抗人CD3单克隆抗体的研究
Reversal of New-onset Autoimmune Diabetes by a Short-time and Low-dose Treatment with Anti-CD3 Monoclonal Antibodies;
抗CD3单克隆抗体治疗Ⅰ型糖尿病及机制的研究
Optimization of Serum-free Culture System for CHO Cell with Stable Production of Anti-CD3 Humanized Antibody;
抗CD3单克降抗体分泌细胞体外培养条件的优化
Expression, Identification and Bioactivity Characterization of an Anti-CD3/anti-CD20 Bispecific Single-Chain Antibody;
抗CD3/抗CD20双特异性单链抗体的构建、表达及生物学活性检测
Biological Activity Study of a Bispecific-Single-Chain Antibody Against T Cell and Ovarian Carcinoma;
抗人卵巢癌×抗人CD3双特异性单链抗体BHL-1的生物活性研究
Construction and Expression of Multivalent Anti-CD3×anti-prostate-cancer Bispecific Single-Chain Antibody;
多价抗前列腺癌/抗人CD3双特异性单链抗体的构建及表达
Development and Characterization of Monoclonal Antibodies Against Chicken CD3 and IL-2;
鸡CD3和IL-2分子单克隆抗体的研制与鉴定
Amplification, Cloning and Sequence Analysis of the Genes of V_H and V_K of an Anti-human CD3 Monoclonal Antibody;
抗人CD3单抗重、轻链可变区基因的体外扩增、克隆和序列分析
Apoptosis and autophagy induced by anti-CD3 monoclonal antibody in human peripheral blood CD4~+CD25~+T lymphocytes
抗CD3单克隆抗体对人CD4~+CD25~+T淋巴细胞凋亡和自噬的影响
The Design of a Novel CD3-specific Antibody and Its Function;
新型抗CD3小分子抗体的设计及其功能研究
Construction and expression of disulphide stabilized anti-CD3/anti-Pgp diabody
二硫键稳定的抗CD3/抗Pgp微型双功能抗体的构建、表达及活性测定
Studies on Large-scale Fermentation of Anti-CD3 Antibody s Light Chain in Pichia Pastoris;
抗CD3人鼠嵌合抗体轻链在毕赤酵母中大规模发酵表达的研究
Cloning and Expression of Anti-CD3 Human/Murine Chimeric Antibody in Pichia Pastoris;
抗CD3人鼠嵌合抗体的克隆及其在毕赤酵母中的表达
The Preparation and Study on Anti-human-gastrin McAb and ScFv;
抗人胃泌素单克隆抗体及其单链抗体的研制
Humanization of Mouse Anti-bladder Cancer McAb by CDR3 Directed Phage Antibody Library;
CDR3导向抗体库技术人源化抗膀胱癌单抗BDI