Comparison on five methods for extraction of RNA from Echinococcus multilocularis metacestode Larva and Studies on amplification of DNA long fragments with RT PCR;
5种多房棘球绦虫续绦期幼虫的制备RNA方法比较及长片段DNART-PCR扩增方法研究
Betaine Improves LA-PCR Amplification;
甜菜碱增强长片段PCR的扩增(英文)
Target gene segments were amplified by long reverse transcriptase polymerase chain reaction(LRP).
方法根据基因组中限制性酶切位点的分布设计4对引物,QIAamp RNA抽提试剂盒提取西尼罗病毒培养细胞BHK-21的总RNA,长片段RT-PCR技术扩增目的基因片段。