Application of Detergent/polymer Aqueous Two Phase Systems for Purification of Membrane Protein;
去污剂/聚合物双水相体系在膜蛋白分离中的应用
Methods: Glioma cells were split with two groups of detergent (1)2% Triton X-114;(2)1% SDS, 1% Deoxycholic acid Na salt, 1% NP-40, 1mmol/L EDTA to extract cell proteins respectively, then SDS-PAGE was adopted to separate the proteins and Western-Blot was adopted to identify the specific protein.
方法 :分别以两组去污剂 (1) 2 % Triton X- 114;(2 ) 1% SDS、1%去氧胆酸钠、1% NP- 40、1mmol/L EDTA裂解 SHG- 44细胞 ,提取细胞蛋白 ,再以 SDS- PAGE法分离蛋白 ,并以 Western- Blot法分析鉴定。
However,it is hard to get sufficient high quality protein for crystallization,together with its instability in detergent solution,which had hampered high-resolution structure determination of GPCRs.
近年来,随着新型膜蛋白表达体系、新型去污剂、膜蛋白纯化及结晶技术的发展,使得G蛋白偶联受体结构解析工作取得了可喜的进展,也为进一步解析更多GPCR精细结构及相关药物研发奠定重要基础。
The thylakiod membranes isolated from Undaria pinnatifida and Ulva pertusa were solubilized by different detergents SDS, Dig and Triton X-100 at different concentrations.
以褐藻裙带菜(Undaria pinnatifida)和绿藻石莼(Ulva pertusa)为实验材料,利用SDS,Dig,Triton X-100三种去污剂,分别与叶绿素按不同比例增溶类囊体膜,采用蔗糖密度梯度超速离心的方法分离色素蛋白复合物,蔗糖密度梯度为60%,50%,40%,30%,20%,15%和10%。
The effects of different concentrations of detergents SDS and Triton-X 100(TX) on thylakoid’s membrane structure and function in spinach were studied in this paper.
研究了不同浓度的去污剂十二烷基磺酸钠(SDS)和聚乙二醇辛基苯乙醚(Triton-X 100,TX)对菠菜类囊体膜的结构与功能的影响。
detergency test
脱垢试验,去污试验
Decontamination of radioactivily contaminated surface--Method for testing and assessing the ease of decontamination
GB/T14057-1993放射性污染表面去污试验与评价去污难易程度的方法
Decontamination of radioactively contaminated surfaces Testing of decontamination agents for textiles??
放射性污染表面的去污? 对纺织品去污剂的试验
Decontamination of radioactively contaminated surfaces--Testing method of decontamination agents for textiles
GB/T15850-1995放射性污染表面的去污纺织品去污剂的试验方法
Lab-Scale Study on Removal of Estrogen from Sewage by A~2O Activated Sludge Process
A~2O活性污泥工艺去除污水中雌激素的试验
Study on Heavy Metal Removal from Urban Sewage Sludge and Utilization;
城市污水污泥重金属去除与污泥农用资源化试验研究
Experimental Research on Pollutant Removal from Drinking Water by Ultrafiltration Membrane;
超滤膜去除饮用水中污染物的试验研究
Research on Test of Nitrogen and Phosphor Removal from Sewage by Subsurface Flow Constructed Wetlands;
潜流人工湿地去除生活污水中氮磷的试验研究
Study on Nitrogen and Phosphorus Removal with Attached Algae in Light Polluted Water;
着生藻类去除微污染水体中氮磷的试验研究
Pilot Research on the Removal of Sudden Organic Phosphorus Pesticides Pollution in Source Water
水源水中突发性有机磷农药污染去除试验研究
CHARACTERISTICS OF SEVERAL PLANTS FOR REMOVING NITROGEN AND PHOSPHORUS FROM WASTEWATER IN CONSTRUCTED WETLAND
湿地植物对污水中氮、磷去除效果的试验研究
Research on Removal and Recovery of Heavy Metals from Municipal Sludge
城市污泥中重金属的去除与回收试验研究
Study on the finishing technology of oil resistance, water repellency and soil resistance for tie fabrics
07领带面料抗油、拒水及易去污后整理工艺试验
Study on simultaneous removal of H_2S by biological aerated filter in the course of domestic sewerage treatment
BAF处理生活污水过程中同步去除H_2S的试验研究
Study on electro-kinetic removal of Pb from municipal sewage sludge
动电技术去除城市污泥中Pb的试验研究
Experimental Research on Immobilization of U(Ⅵ) in Solution by Zero-valent Iron and Hematite;
零价铁和赤铁矿去除污染水体中U（Ⅵ）的试验研究
Removal of Bisphenol A (BPA) from Simulated Domestic Wastewater by Membrane Bioreactor (MBR) and Conventional Activated Sludge Reactor (CASR);
膜—生物反应器和普通活性污泥法去除原水中双酚A的试验研究
Research on Removal of Organic Pollutant in Drinking Water by Zeolite-Active Carbon;
沸石—活性炭去除饮用水中有机污染物的试验研究