Standardization of restriction endonuclease expression in molecular biology books and periodicals;
分子生物学书刊中限制性内切酶的规范表达
Choice of restriction endonuclease and establishment of silver-staining method in cDNA-AFLP System for Carthamus tinctorius
红花cDNA-AFLP限制性内切酶的选择及银染体系的建立
The plasmid DNA of the 20 strains were not only isolated but also digested by restriction endonuclease and agarose gel electrophoresis.
从含KCN的分离筛选培养基上分离出的 2 0株菌株进行了质粒抽提 ,对质粒进行了限制性内切酶的酶切和琼脂糖凝胶电泳 ,发现了 1 1株细菌带有质粒 ,对其中几株带质粒菌和不带质粒菌进行了降氰力的测定 ,说明了质粒对降氰能力具有重要意义 ,并对 6株菌测定了质粒分子量 ,但未发现降氰力与分子量间的相关性。
The Analysis of the restriction enzyme of mitochondrial DNA of Heilongjiang Carp;
黑龙江野鲤线粒体DNA限制性内切酶酶切分析
The diagnostic value of restriction enzyme analysis in virosis;
限制性内切酶分析法对病毒感染性疾病诊断的意义
In recent years,as a new way,restriction enzyme-mediated integration(REMI) has been developed to study genes.
限制性内切酶介导的整合技术是近年发展起来的一种研究基因的新方法,与质粒拯救相结合,可快速分离质粒两侧的DNA序列,进而对该序列做进一步分析和研究。
First, genomic DNA is digested with different restriction endonucleases, including blunt-end and sticky-end ones.
利用染色体步行法,从已知DNA序列克隆侧翼未知序列是非常有效的方法之一,但由于所选用的特定限制性内切酶对目标基因组不能酶解成合适大小的片段,因而受PCR扩增能力的局限,往往扩增不出有效产物。
A total of 12 restriction endonucleases were used to analyse in mtDNA of the cytochrome b(Cytb) gene within 6 indigenous sheep breeds.
用12种限制性内切酶分析了6个地方绵羊品种线粒体DNA(m tDNA)细胞色素b(cytb)基因的RFLP。
A total of 22 restriction endonucleases were used to investigate the mitochondrial DNA polymorphism of Sanjiang White pigs.
采用 2 2种限制性内切酶分析了三江白猪线粒体DNA的多态性。
Methods The restriction endonuclease and T4 DNA ligase were used to construct the vector plasmid.
方法载体的构建采用限制性内切酶酶切、4DNA连接酶连接等方法。
Research of Reaction Mechanism and Kinetics Analysis for the FokI Restriction Endonuclease;
限制性内切酶FokI作用机制研究及动力学分析
Secondly, special enzymes called extinction enzymes are used to cut the DNA at specific places.
第二，利用被叫做限制性内切酶的特殊的酶在特定地方切断DNA。
Large DNA molecules are first dissected with restriction enzymes to produce specific fragments.
首先用限制性内切酶将大的DNA分子切断，产生出特殊的片段。
It enables a specific gene to be located on a particular restriction enzyme fragment.
它就能使专一的基因被定位于特定的限制性内切酶切成的片段上。
Development and Clinical Application of SARS Virus cDNA Restriction Endoenzyme Analysis Kit;
SARS病毒cDNA限制性内切酶检测试剂盒的研制和临床应用研究
A Novel Fluorescence Assay for the Activity of Restriction Endonuclease Based on Molecular Beacon
分子信标检测限制性内切酶活性的新型荧光分析方法
Construction of Protoplast-Based Transformation System of Fungal Biocontrol Agent Paecilomyces Fumosoroseus with Restriction Enzyme-Mediated Integration (REMI);
限制性内切酶介导的玫烟色拟青霉原生质转化体系构建
The Restriction Endonuclease Analysis and Elimination of pR_(st98) in Salmonella typhi;
伤寒杆菌质粒pR_(st98)的DNA限制性内切酶分析和药物的体外消除研究
Inserter Mutation by Restriction Enzyme-mediated Integration and Agrobacterium tumefaciens-mediated in Trichoderma koningii and Isolation of the Mutant
限制性内切酶和农杆菌介导的康宁木霉插入突变及突变体的筛选
Choice of restriction endonuclease and establishment of silver-staining method in cDNA-AFLP System for Carthamus tinctorius
红花cDNA-AFLP限制性内切酶的选择及银染体系的建立
The Design of Novel Molecular Probes and Their Applications in Real Time Assays for Methylase & Restriction Endonuclease and Drug-screening;
新型分子探针的设计及其在甲基化酶和限制性内切酶实时分析及药物筛选中的应用
Results The expressive vector has been constructed and confirmed by restriction enzyme digestion and DNA sequencing analysis.
结果构建的表达载体经过限制性内切酶分析和DNA序列测定,证实准确无误。
Genetic Transformation and Phenotypic Analysis of Transformants of REMI-Mediated Exserohilum Turcicum;
限制性内切酶介导（REMI）玉米大斑病菌的遗传转化及其转化子表型分析
Studies on the Active Groups of Pst I Restriction Endonuclease
限制性核酸内切酶PstⅠ活性基团的研究
circular restriction map
环形限制（酶切）图（谱）
The distribution of polymorphism at restriction enzyme site-48 A/ G was analyzed with the method of polymerase chain reaction-restriction fragment length polymorphism( PCR-RFLP).
用聚合酶链反应-限制性片段长度多态性方法,分析限制性酶切位点-48A/的多态分布。
Identification of Thermus to the Species Level using 16S rDNA Gene Restriction Fragment Patterns
利用针对16S rDNA序列的限制性酶切分析鉴定栖热菌属